Campus Units
Animal Science
Document Type
Article
Publication Version
Published Version
Publication Date
11-1-2006
Journal or Book Title
Poultry Science
Volume
85
Issue
11
First Page
1907
Last Page
1911
DOI
10.1093/ps/85.11.1907
Abstract
Cytokines are secreted proteins involved with cell recruitment and regulation of both innate and adaptive immune responses. They are essential for an effective host immune response to pathogens. The objective of this study was to determine the effect of Salmonella enterica serovar Enteritidis (S. Enteritidis) exposure and genetic line on cytokine mRNA expression level of cultured chicken peripheral blood mononuclear cells (PBMC). Interleukin-2, interleukin-6 (IL-6), CXCLi2, and transforming growth factor-β4 (TGF-B4) messenger ribonucleic acid expression was measured by quantitative reverse transcription-PCR assays in PBMC from 3 chicken lines (broiler, Leghorn, Fayoumi) after in vitro exposure to S. Enteritidis. The PBMC were isolated from uninfected birds and cultured overnight. The next day, live pathogenic S. Enteritidis was added to half of the cultures. All cultures were harvested after 2 or 4 h of exposure. Exposure to S. Enteritidis downregulated IL-6, CXCLi2, and TGF-β4 but not interleukin-2 mRNA expression. No significant genetic line or exposure time effects were detected. These findings demonstrate that exposure of chicken PBMC to S. Enteritidis can induce a rapid change in both proinflammatory (IL-6, CXCLi2) and antiinflammatory (TGF-β4) cytokine gene expression.
Creative Commons License
This work is licensed under a Creative Commons Attribution-Noncommercial-No Derivative Works 4.0 License.
Copyright Owner
Poultry Science Association Inc.
Copyright Date
2006
Language
en
File Format
application/pdf
Recommended Citation
Kaiser, Michael G.; Cheeseman, Jennifer H.; Kaiser, P.; and Lamont, Susan J., "Cytokine Expression in Chicken Peripheral Blood Mononuclear Cells after In Vitro Exposure to Salmonella enterica serovar Enteritidis" (2006). Animal Science Publications. 605.
https://lib.dr.iastate.edu/ans_pubs/605
Comments
This article is published as Kaiser, M. G., J. H. Cheeseman, P. Kaiser, and S. J. Lamont. "Cytokine expression in chicken peripheral blood mononuclear cells after in vitro exposure to Salmonella enterica serovar Enteritidis." Poultry Science 85, no. 11 (2006): 1907-1911. DOI: 10.1093/ps/85.11.1907. Posted with permission.