Campus Units

Animal Science

Document Type

Article

Publication Version

Published Version

Publication Date

1-1-2008

Journal or Book Title

Poultry Science

Volume

87

Issue

1

First Page

22

Last Page

26

DOI

10.3382/ps.2007-00259

Abstract

Salmonella enteritidis lipopolysaccharide stimulates interleukin 10 (IL10) gene expression in chickens. Four genes in the IL10 cluster [polymeric immunoglobulin receptor (PIGR), interleukin 10 (IL10), map kinase-activated protein kinase 2 (MAPKAPK2), and ligatin (LGTN)] plus dual-specificity tyrosine-(Y)-phosphorylation regulated kinase1A (DYRK1A) were investigated using the F8 generation of 2 related advanced intercross lines (AIL). The AIL were generated by crossing outbred broilers with dams of 2 highly inbred lines (Leghorn and Fayoumi). Intercrossing continued within the 2 dam lines. The F8 chicks (n = 132) were intraesophageally inoculated at 1 d with S. enteritidis. At d 7 or 8, both spleen tissue and cecal contents were cultured to quantify S. enteritidis load. The F8 population was genotyped for one single nucleotide polymorphism (SNP) per gene by using a multiplexed SNaPshot assay. Association of gene SNP with S. enteritidis bacterial burden was analyzed by the GLM. The MAPKAPK2 and IL10 genes were highly (P < 0.001) associated with S. enteritidis burden in spleen tissue and cecal luminal content. Suggestive associations (P < 0.05) of PIGR with spleen tissue and cecal content were found. The results suggest that SNP in MAPKAPK2 and IL10 were strongly associated with Salmonella burden and may be valuable in generating resistant birds by marker-assisted selection.

Comments

This article is published as Ghebremicael, S. B., J. R. Hasenstein, and S. J. Lamont. "Association of interleukin-10 cluster genes and Salmonella response in the chicken." Poultry Science 87, no. 1 (2008): 22-26. DOI: 10.3382/ps.2007-00259. Posted with permission.

Copyright Owner

Poultry Science Association Inc.

Language

en

File Format

application/pdf

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