Campus Units
Animal Science
Document Type
Article
Publication Version
Published Version
Publication Date
4-1-2004
Journal or Book Title
Poultry Science
Volume
83
Issue
4
First Page
544
Last Page
549
DOI
10.1093/ps/83.4.544
Abstract
Salmonella enteritidis is a foodborne pathogen that negatively affects both animal and human health. Genetic variations in response to pathogenic SE colonization or to SE vaccination were measured in a chicken resource population. Outbred broiler sires and 3 diverse, highly inbred dam lines produced 508 F1 progeny that were evaluated for either bacterial colonization after pathogenic SE inoculation or circulating antibody level after SE vaccination. Five candidate genes were selected for study, based on their biological function as possibly affecting response to SE: toll-like receptor 4 (TLR4), T-cell specific surface protein (CD28), macrophage migration inhibitory factor (MIF), MD-2, and lipopolysaccharide-induced tumor necrosis factor (TNF)-α factor (LITAF). Gene fragments were sequenced from the founder lines of the resource population. The LITAF and MIF genes were homozygous for all sires. Single nucleotide polymorphisms (SNP) were identified in 3 genes (TLR4, CD28, and MD-2) and were used to test for associations of sire SNP with SE response. Linear mixed models were used for statistical analyses. The CD28 broiler sire SNP was associated with both bacterial load in the cecum (P < 0.003) and vaccine antibody response (P < 0.05). The MD-2 SNP was associated (P < 0.04) with the bacterial load in the spleen. The use of these SNP in these genes in marker-assisted selection may result in enhancement of disease resistance.
Creative Commons License
This work is licensed under a Creative Commons Attribution-NonCommercial-No Derivative Works 4.0 International License.
Copyright Owner
Poultry Science Association Inc.
Copyright Date
2004
Language
en
File Format
application/pdf
Recommended Citation
Malek, M.; Hasenstein, J. R.; and Lamont, Susan J., "Analysis of Chicken TLR4, CD28, MIF, MD-2, and LITAF Genes in a Salmonella enteritidis Resource Population" (2004). Animal Science Publications. 614.
https://lib.dr.iastate.edu/ans_pubs/614
Comments
This article is published as Malek, M., J. R. Hasenstein, and S. J. Lamont. "Analysis of chicken TLR4, CD28, MIF, MD-2, and LITAF genes in a Salmonella enteritidis resource population." Poultry Science 83, no. 4 (2004): 544-549. DOI: 10.1093/ps/83.4.544. Posted with permission.