Campus Units
Animal Science
Document Type
Article
Publication Version
Published Version
Publication Date
6-1-2004
Journal or Book Title
Poultry Science
Volume
83
Issue
6
First Page
911
Last Page
916
DOI
10.1093/ps/83.6.911
Abstract
To determine the role of genetics in baseline lymphocyte parameters, several distinct lines of chickens were examined for differences in peripheral blood leukocyte (PBL) populations. Four highly inbred chicken lines (MHC congenic Fayoumi lines M15.2 and M5.1, and MHC congenic Leghorn lines G-B1 and G-B2), two advanced intercrosses [F5 (Broiler × G-B2) and F5 (Broiler × M15.2)], and an outbred population of broilers were used. Leukocytes isolated from healthy adult birds were labeled with monoclonal antibodies: chCD3, chCD4, chCD8, chBu-1, and hCD14. Flow cytometry was used to determine the total percentage of positively labeled cells for each surface marker in a sample, as well as the mean fluorescent intensity, or surface marker density, of a labeled subset. Significant line differences for percentage positive CD3 T cells and the ratio of B cells:T cells (represented by the Bu-1:CD3 ratio) were found. The effect of line was also significant for CD3 and CD8 T cell receptor density. Effects of sex and MHC on PBL cell surface marker expression were not significant in the lines examined. This study demonstrates the effect of genetic line on resting leukocyte composition of peripheral blood in the chicken lines examined. Observed PBL differences add to our growing knowledge of the varied roles that immune system status (defined by specific cell populations) and genetic background have in determining susceptibility and disease progression in chickens.
Creative Commons License
This work is licensed under a Creative Commons Attribution-NonCommercial-No Derivative Works 4.0 International License.
Copyright Owner
Poultry Science Association, Inc.
Copyright Date
2004
Language
en
File Format
application/pdf
Recommended Citation
Cheeseman, J. H.; Kaiser, Michael G.; and Lamont, Susan J., "Genetic Line Effect on Peripheral Blood Leukocyte Cell Surface Marker Expression in Chickens" (2004). Animal Science Publications. 615.
https://lib.dr.iastate.edu/ans_pubs/615
Comments
This article is published as Cheeseman, J. H., M. G. Kaiser, and S. J. Lamont. "Genetic line effect on peripheral blood leukocyte cell surface marker expression in chickens." Poultry Science 83, no. 6 (2004): 911-916. DOI: 10.1093/ps/83.6.911. Posted with permission.