Campus Units
Animal Science
Document Type
Article
Publication Version
Published Version
Publication Date
5-2014
Journal or Book Title
PLoS ONE
Volume
9
Issue
5
First Page
e97567
DOI
10.1371/journal.pone.0097567
Abstract
Alpha-dystroglycan requires a rare O-mannose glycan modification to form its binding epitope for extracellular matrix proteins such as laminin. This functional glycan is disrupted in a cohort of muscular dystrophies, the secondary dystroglycanopathies, and is abnormal in some metastatic cancers. The most commonly used reagent for detection of alpha-dystroglycan is mouse monoclonal antibody IIH6, but it requires the functional O-mannose structure for recognition. Therefore, the ability to detect alpha-dystroglycan protein in disease states where it lacks the full O-mannose glycan has been limited. To overcome this hurdle, rabbit monoclonal antibodies against the alpha-dystroglycan C-terminus were generated. The new antibodies, named 5–2, 29–5, and 45–3, detect alpha-dystroglycan from mouse, rat and pig skeletal muscle by Western blot and immunofluorescence. In a mouse model of fukutin-deficient dystroglycanopathy, all antibodies detected low molecular weight alpha-dystroglycan in disease samples demonstrating a loss of functional glycosylation. Alternately, in a porcine model of Becker muscular dystrophy, relative abundance of alpha-dystroglycan was decreased, consistent with a reduction in expression of the dystrophin-glycoprotein complex in affected muscle. Therefore, these new rabbit monoclonal antibodies are suitable reagents for alpha-dystroglycan core protein detection and will enhance dystroglycan-related studies.
Rights
Works produced by employees of the U.S. Government as part of their official duties are not copyrighted within the U.S. The content of this document is not copyrighted.
Language
en
File Format
application/pdf
Recommended Citation
Fortunato, Marisa J.; Ball, Charlotte E.; Hollinger, Katrin; Patel, Niraj B.; Modi, Jill N.; Rajasekaran, Vedika; Nonneman, Dan J.; Ross, Jason W.; Kennedy, Eileen J.; Selsby, Joshua T.; and Beedle, Aaron M., "Development of Rabbit Monoclonal Antibodies for Detection of Alpha-Dystroglycan in Normal and Dystrophic Tissue" (2014). Animal Science Publications. 691.
https://lib.dr.iastate.edu/ans_pubs/691
Included in
Agriculture Commons, Animal Experimentation and Research Commons, Animal Sciences Commons, Diseases Commons
Comments
This article is published as Fortunato MJ, Ball CE, Hollinger K, Patel NB, Modi JN, Rajasekaran V, et al. (2014) Development of Rabbit Monoclonal Antibodies for Detection of Alpha-Dystroglycan in Normal and Dystrophic Tissue. PLoS ONE 9(5): e97567. doi: 10.1371/journal.pone.0097567.