Campus Units
Animal Science
Document Type
Article
Publication Version
Published Version
Publication Date
8-2017
Journal or Book Title
Physiological Reports
Volume
5
Issue
16
First Page
e13397
DOI
10.14814/phy2.13397
Abstract
Despite well‐studied clinical manifestations, intracellular mechanisms of prolonged hyperthermic injury remain unclear, especially in skeletal muscle. Given muscle's large potential to impact systemic inflammation and metabolism, the response of muscle cells to heat‐mediated injury warrants further investigation. We have previously reported increased activation of NF‐κB signaling and increased NF‐κB and AP‐1‐driven transcripts in oxidative skeletal muscle following 12 h of heat stress. The purpose of this investigation was to examine early heat stress‐induced inflammatory signaling in skeletal muscle. We hypothesized that heat stress would increase NF‐κB and AP‐1 signaling in oxidative skeletal muscle. To address this hypothesis, 32 gilts were randomly assigned to one of four treatment groups (n = 8/group): control (0 h: 21°C) or exposed to heat stress conditions (37°C) for 2 h (n = 8), 4 h (n = 8), or 6 h (n = 8). Immediately following environmental exposure pigs were euthanized and the red portion of the semitendinosus muscle (STR) was harvested. We found evidence of NF‐κB pathway activation as indicated by increased protein abundance of NF‐κB activator IKK‐α following 4 h and increased total NF‐κB protein abundance following 6 h of heat stress. Heat stress also stimulated AP‐1 signaling as AP‐1 protein abundance was increased in nuclear fractions following 4 h of heat stress. Interleukin‐6 protein abundance and activation of the JAK/STAT pathway were decreased in heat stressed muscle. These data indicate that heat stress activated inflammatory signaling in the porcine STR muscle via the AP‐1 pathway and early activation of the NF‐κB pathway.
Creative Commons License
This work is licensed under a Creative Commons Attribution 4.0 License.
Copyright Owner
The Authors
Copyright Date
2017
Language
en
File Format
application/pdf
Recommended Citation
Ganesan, Shanthi; Volodina, Olga; Pearce, Sarah C.; Gabler, Nicholas K.; Baumgard, Lance H.; Rhoads, Robert P.; and Selsby, Joshua T., "Acute heat stress activated inflammatory signaling in porcine oxidative skeletal muscle" (2017). Animal Science Publications. 697.
https://lib.dr.iastate.edu/ans_pubs/697
Included in
Animal Experimentation and Research Commons, Animal Sciences Commons, Musculoskeletal, Neural, and Ocular Physiology Commons
Comments
This article is published as Ganesan, Shanthi, Olga Volodina, Sarah C. Pearce, Nicholas K. Gabler, Lance H. Baumgard, Robert P. Rhoads, and Joshua T. Selsby. "Acute heat stress activated inflammatory signaling in porcine oxidative skeletal muscle." Physiological reports 5, no. 16 (2017): e13397. doi: 10.14814/phy2.13397.