Campus Units

Animal Science

Document Type

Article

Publication Version

Published Version

Publication Date

5-2010

Journal or Book Title

The Journal of Physiological Sciences

Volume

60

First Page

181

Last Page

186

DOI

10.1007/s12576-009-0082-2

Abstract

Increased myocardial cyclooxygenase-2 (COX-2) activity is essential for late phase ischemic preconditioning (IPC). Currently unknown is whether cardioprotection elicited by exercise also involves elevated myocardial COX-2 activity. This investigation tested whether aerobic exercise elevates myocardial COX-2 protein content or enzyme activity in young and senescent male Fisher 344 rats assigned to sedentary or cardioprotective endurance exercise treatments (3 consecutive days of treadmill exercise, 60 min/day @~70% VO2max). Assay of cardiac COX-2 protein content, catalytic activity, and inducible nitric oxide synthase (iNOS) protein content reveal that exercise did not alter COX-2 activity (PGE2, p = 0.866; PGF1α, p = 0.796) or protein levels (p = 0.397) within young or senescent hearts. In contrast, myocardial iNOS, an up-stream mediator of COX-2 expression, was over-expressed by an average of 37% in aged hearts (p = 0.005), though iNOS was not influenced by exercise. Findings reveal exercise does not elevate cardiac COX-2 activity and suggests that mechanisms responsible for cardioprotection differ between IPC and aerobic exercise.

Comments

This article is published as Quindry, J.C., French, J., Hamilton, K.L. et al. Exercise does not increase cyclooxygenase-2 myocardial levels in young or senescent hearts. J Physiol Sci 60, 181–186 (2010). doi: 10.1007/s12576-009-0082-2.

Creative Commons License

Creative Commons Attribution 4.0 License
This work is licensed under a Creative Commons Attribution 4.0 License.

Copyright Owner

The Physiological Society of Japan and Springer

Language

en

File Format

application/pdf

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