Campus Units
Animal Science
Document Type
Article
Publication Version
Published Version
Publication Date
3-1-2001
Journal or Book Title
Poultry Science
Volume
8
Issue
3
First Page
284
Last Page
288
DOI
10.1093/ps/80.3.284
Abstract
Thirteen gene-specific primer sets provided by the U.S. Poultry Genome Coordinators were used to investigate DNA polymorphisms between two highly inbred chicken lines of Leghorn and Fayoumi origin. Nucleotide and predicted amino acid sequences were then compared among these chicken lines and the Genbank sequences of chicken, mouse, and human. The following genes were selected as candidates for immune response or transcription activation: B2M, DAD1, IAP1, IL2, IREB1, LAP18, MAFL, POU1F1, RREB1, TAD, TBP1, TCRG, and ZOV3. Total cDNA was obtained from the spleens of Leghorn and Fayoumi lines by reverse transcriptase-polymerase chain reaction (PCR) and was used as a template to PCR-amplify gene-specific products. All primers except POU1F1 and TCRG generated single PCR products of the predicted 325- to 667-bp size, confirming the efficacy of these gene-specific primers in the chicken. Three and seven of the 11 amplified gene fragments yielded line-specific nucleotide polymorphisms between the Leghorn and Fayoumi sequences and between the Leghorn and Genbank chicken sequences respectively. Similarities between inbred Leghorn and mammalian species were 36 to 86% for nucleotides and 25 to 96% for predicted amino acid sequence. The polymorphisms of some gene fragments between the Leghorn and Fayoumi lines will allow for investigation of associations of these genes with immune response and other biological traits.
Creative Commons License
This work is licensed under a Creative Commons Attribution-NonCommercial-No Derivative Works 4.0 International License.
Copyright Owner
Poultry Science Association Inc.
Copyright Date
2001
Language
en
File Format
application/pdf
Recommended Citation
Zhou, H.; Liu, W.; and Lamont, Susan J., "Genetic Variation Among Chicken Lines and Mammalian Species in Specific Genes" (2001). Animal Science Publications. 718.
https://lib.dr.iastate.edu/ans_pubs/718
Included in
Agriculture Commons, Genetics Commons, Molecular Biology Commons, Poultry or Avian Science Commons
Comments
This article is published as Zhou, Huaijun, Wei Liu, and Susan J. Lamont. "Genetic variation among chicken lines and mammalian species in specific genes." Poultry Science 80, no. 3 (2001): 284-288. DOI: 10.1093/ps/80.3.284. Posted with permission.