Campus Units

Animal Science

Document Type

Article

Publication Version

Published Version

Publication Date

12-1-2001

Journal or Book Title

Poultry Science

Volume

80

Issue

12

First Page

1679

Last Page

1689

DOI

10.1093/ps/80.12.1679

Abstract

An F2 population was produced from mating G0 highly inbred (>99%) males of two MHC-congenic Fayoumi lines with G-B1 Leghorn hens. The F2 population was essentially a full-sibship with the F1 sire line reflecting MHC effect. Adult F2 hens (n = 158) were injected twice with SRBC and whole fixed Brucella abortus (BA). Agglutinating antibody titer at 7 d after primary immunization and mean titer of the final three samples (Days 18, 32, and 63 after the second immunization) were used as parameters for primary and equilibrium phases, respectively. Secondary phase parameters of minimum (Ymin), maximum titers (Ymax) and time needed to achieve minimum (Tmin) and maximum (Tmax) titers were estimated from seven postsecondary titers with a nonlinear regression model. Three candidate genes, interferon-γ (IFN-γ), interleukin-2 (IL-2), and immunoglobulin G light chain (IgL) were studied. Primers for the promoter regions were designed from EMBL chicken genomic sequences. Polymorphisms between parental lines were detected by direct sequencing. Polymerase chain reaction-restriction fragment length polymorphism methods were then developed to directly detect the polymorphism. There were significant main effects (P < 0.05, general linear model analysis) of IFN-γ polymorphism on Ymax of BA antibody and interaction of IFN-γ by IgL on primary antibody response to SRBC and BA, and on Tmin and Ymin of antibody response to SRBC in F2 offspring of M5.1 grandsires. There were significant main effects of IFN-γ polymorphism on Tmax of BA and interaction of IFN-γ by IL-2 on Ymin to SRBC in F2 offspring of M15.2 grandsires. The results suggest that IFN-γ genes play an important role in chicken primary and secondary antibody response to SRBC and BA antigens, and there exists interaction among genes for antibody production.

Comments

This article is published as Zhou, Huaijun, A. J. Buitenhuis, Steffen Weigend, and S. J. Lamont. "Candidate gene promoter polymorphisms and antibody response kinetics in chickens: interferon-γ, interleukin-2, and immunoglobulin light chain." Poultry Science 80, no. 12 (2001): 1679-1689. DOI: 10.1093/ps/80.12.1679. Posted with permission.

Creative Commons License

Creative Commons Attribution-Noncommercial-No Derivative Works 4.0 License
This work is licensed under a Creative Commons Attribution-Noncommercial-No Derivative Works 4.0 License.

Copyright Owner

Poultry Science Association Inc.

Language

en

File Format

application/pdf

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