Campus Units

Animal Science

Document Type

Article

Publication Version

Accepted Manuscript

Publication Date

4-3-2021

Journal or Book Title

Livestock Science

First Page

104503

DOI

10.1016/j.livsci.2021.104503

Abstract

We investigated how feed restriction at 50% of maintenance requirements during different stages of gestation affects the transcriptome of newborn goats’ skeletal muscle. Fourteen pregnant dams were randomly assigned into one of the following dietary treatments: animals fed at 50% of maintenance requirement from 8-84 d of gestation and then fed at 100% of maintenance requirement from day 85 of gestation to parturition (RM, n = 6), and animals fed at 100% of maintenance requirement from 8-84 d of gestation and then fed at 50% of maintenance requirement from day 85 of gestation to parturition (MR, n = 8). At birth, samples of offspring’s Longissimus muscle were collected for total RNA extraction and sequencing. Our data showed 66 differentially expressed (DE) genes (FDR < 0.05). A total of 6 genes were upregulated and 60 downregulated (FDR < 0.05) in the skeletal muscle of the newborns resulting from treatment RM compared with MR. Our results suggest that the DE genes upregulated in newborn goats’ skeletal muscle from the RM group compared to MR, included genes related to satellite cells, and genes that indicates impaired insulin sensitivity and changes in the composition of intramuscular fat. The DE genes upregulated in newborn goats’ skeletal muscle from the MR group compared to RM, are also related to impaired insulin sensitivity, as well as a predominantly oxidative metabolism and cellular oxidative stress. However, protective mechanisms against insulin sensitivity and oxidative stress may have been augmented in the skeletal muscle of offspring from MR treatment compared to RM, in order to maintain cellular homeostasis.

Comments

This is a manuscript of an article published as Costa, Thaís C., Tiago AO Mendes, Marta MS Fontes, Mariana M. Lopes, Min Du, Nick VL Serão, Leticia MP Sanglard et al. "Transcriptome changes in newborn goats’ skeletal muscle as a result of maternal feed restriction at different stages of gestation." Livestock Science (2021): 104503. doi:10.1016/j.livsci.2021.104503. Posted with permission.

Copyright Owner

Elsevier B.V.

Language

en

File Format

application/pdf

Available for download on Sunday, April 03, 2022

Published Version

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