Campus Units

Animal Science

Document Type

Article

Publication Version

Published Version

Publication Date

6-1-1992

Journal or Book Title

Poultry Science

Volume

71

Issue

6

First Page

999

Last Page

1006

DOI

10.3382/ps.0710999

Abstract

Selected chicken populations were analyzed by restriction fragment length polymorphism (RFLP) with a chicken MHC Class I (B-F) cDNA probe. The 13 highly inbred chicken lines differed in genetic origin and in MHC (B) haplotype, as distinguished by using hemagglutination with antisera against B-G and B-F antigens. The S1 sublines differed for B haplotype and antibody response to a synthetic polypeptide, GAT. In the highly inbred lines, band-sharing between lines from different origins was less than that between lines from same origin, showing the influence of the genetic background on chicken MHC Class I gene RFLP. In the S1 line, use of three restriction endonucleases (BglII, PvuII, and TaqI) produced MHC Class I RFLP that were associated with B haplotype, but not with immune response to GAT (IrGAT). A previous study in the authors' laboratory also demonstrated an association of MHC Class IIβ RFLP patterns with B haplotype, but not IrGAT, in the same line, suggesting that IrGAT is not controlled by MHC Class I or Class IIβ genes.

Comments

This article is published as Chen, Y., and S. J. Lamont. "Major histocompatibility complex class I restriction fragment length polymorphism analysis in highly inbred chicken lines and lines selected for major histocompatibility complex and immunoglobulin production." Poultry Science 71, no. 6 (1992): 999-1006. DOI: 10.3382/ps.0710999. Posted with permission.

Copyright Owner

Poultry Science Association Inc.

Language

en

File Format

application/pdf

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