Campus Units

Animal Science, Biochemistry, Biophysics and Molecular Biology, Roy J. Carver Department of

Document Type

Article

Publication Version

Published Version

Publication Date

1-1-1989

Journal or Book Title

Poultry Science

Volume

68

Issue

12

First Page

94

Last Page

99

DOI

10.3382/ps.0680094

Abstract

Sperm DNA was isolated from chickens of the Iowa State University S1 line. Birds were from sublines selected for B-G antigen, humoral immune response to glutamic acid-alanine-tyrosine (IrGAT), and response to Rous sarcoma virus-induced (RSV) tumors. The DNA was digested with restriction enzymes and subjected to Southern blot analysis with a DNA probe specific for a class II gene of the chicken major histocompatibility complex (MHC).

Restriction fragment length polymorphisms (RFLP) associated with B-G antigen type were found after digestion of DNA with three enzymes, PvuII, BglII, or Sau3A, out of a total of 15 tested. No RFLP were shown to be associated with IrGAT or RSV type. This study shows that RFLP analysis of DNA may be a useful addition to or alternative to serological evaluation of MHC haplotype in the chicken.

Comments

This article is published as Pitcovski, J., S. J. Lamont, A. W. Nordskog, and C. M. Warner. "Analysis of BG and immune response genes in the Iowa State University S1 chicken line by hybridization of sperm deoxyribonucleic acid with a major histocompatibility complex class II probe." Poultry Science 68, no. 1 (1989): 94-99. DOI: 10.3382/ps.0680094. Posted with permission.

Copyright Owner

Poultry Science Association Inc.

Language

en

File Format

application/pdf

Share

COinS