Animal Science, Biochemistry, Biophysics and Molecular Biology, Roy J. Carver Department of
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Sperm DNA was isolated from chickens of the Iowa State University S1 line. Birds were from sublines selected for B-G antigen, humoral immune response to glutamic acid-alanine-tyrosine (IrGAT), and response to Rous sarcoma virus-induced (RSV) tumors. The DNA was digested with restriction enzymes and subjected to Southern blot analysis with a DNA probe specific for a class II gene of the chicken major histocompatibility complex (MHC).
Restriction fragment length polymorphisms (RFLP) associated with B-G antigen type were found after digestion of DNA with three enzymes, PvuII, BglII, or Sau3A, out of a total of 15 tested. No RFLP were shown to be associated with IrGAT or RSV type. This study shows that RFLP analysis of DNA may be a useful addition to or alternative to serological evaluation of MHC haplotype in the chicken.
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Pitcovski, J.; Lamont, Susan J.; Nordskog, A. W.; and Warner, C. M., "Analysis of B-G and Immune Response Genes in the Iowa State University S1 Chicken Line by Hybridization of Sperm Deoxyribonucleic Acid with a Major Histocompatibility Complex Class II Probe" (1989). Animal Science Publications. 848.