Campus Units

Animal Science, Veterinary Pathology

Document Type

Article

Publication Version

Published Version

Publication Date

5-1-1989

Journal or Book Title

Poultry Science

Volume

68

Issue

5

First Page

646

Last Page

650

DOI

10.3382/ps.0680646

Abstract

Complement activity in turkey serum was examined by using inhibitors or activators of mammalian complement. Hemolytic test systems using sheep red blood cells sensitized with specific antibody (SSRBC) and horse red blood cells (HRBC) were developed to measure residual complement activity of turkey sera treated with the various inhibitors or activators. Lysis of SSRBC was blocked by treatment with 6-mM EDTA, 10-mM ethylene glycol-bis-βaminoethylether N,N,N′,N′ tetraacetic acid (EGTA), and carrageenan. In contrast, lysis of HRBC was blocked by 6-mM EDTA, but not by 10-mM EGTA or carrageenan. Addition of magnesium to EGTA-chelated serum facilitated the lysis of HRBC but not the lysis of SSRBC. Treatment of serum with zymosan at 1 mg/mL and inulin at 5 mg/mL depleted hemolytic activity against both SSRBC and HRBC, suggesting depletion of components common to both pathways. Differences in the hemolytic activities of sera against SSRBC and HRBC after treatment with the various complement inhibitors and activators demonstrate the presence of two complement pathways in turkeys.

Comments

This article is published as Ellis, M. G., S. J. Lamont, and L. H. Arp. "Characterization of complement activity in turkeys: evidence for classical and alternative complement pathways." Poultry Science 68, no. 5 (1989): 646-650. DOI: 10.3382/ps.0680646. Posted with permission.

Copyright Owner

Poultry Science Association Inc.

Language

en

File Format

application/pdf

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