Campus Units

Animal Science

Document Type

Article

Publication Version

Published Version

Publication Date

2021

Journal or Book Title

Journal of Veterinary Medicine and Animal Health

Volume

13

Issue

2

First Page

98

Last Page

105

DOI

10.5897/JVMAH2021.0912

Abstract

This study is aimed at evaluating antibody responses, viral loads, viral clearance and growth rate of Tanzanian free-range local chicken (FRLC) challenged with LaSota strain of Newcastle disease virus (NDV) as indicator traits for selection of chickens for breeding with enhanced resistance to the disease and economic value. Three popular free-range local chicken ecotypes: Kuchi, Ching’wekwe and Morogoro-medium from three ecological zones of Tanzania were used for the experiments. Progenies from the breeder chickens were challenged with 107 titer of 50% egg infectious dose (EID50) of the virus at 28 days of age. The viral loads and viral clearance rates evaluated by qRT-PCR from tear samples collected at 2- and 6-days post infection (dpi) showed that Kuchi could clear NDV better than Morogoro-medium and Ching’wekwe. Anti-NDV antibody levels determined from blood samples collected at 10 dpi using ELISA showed that Kuchi ecotype expressed higher mean anti-NDV antibodies compared to Morogoro-medium and Ching’wekwe. Growth rates determined from body weights collected for 38 days from day of hatch (D0) to 10 dpi showed higher growth rate for Kuchi than Morogoro-medium and Ching’wekwe chickens. Kuchi chickens were potentially more resistant to ND compared to Morogoro-medium and Ching’wekwe.

Comments

This article is published as Mushi, James Richard, Gaspar Honorati Chiwanga, Esther Lemburis Mollel, Muhammed Walugembe, Robert Arsen Max, Peter Makenga Msoffe, Rodrigo Gallardo et al. "Antibody response, viral load, viral clearance and growth rate in Tanzanian free-range local chickens infected with lentogenic Newcastle disease virus." Journal of Veterinary Medicine and Animal Health 13, no. 2 (2021): 98-105. doi:10.5897/JVMAH2021.0912.

Creative Commons License

Creative Commons Attribution 4.0 International License
This work is licensed under a Creative Commons Attribution 4.0 International License.

Copyright Owner

Author(s)

Language

en

File Format

application/pdf

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