Campus Units

Animal Science, Food Science and Human Nutrition

Document Type

Article

Publication Version

Published Version

Publication Date

12-1-2008

Journal or Book Title

Poultry Science

Volume

87

Issue

12

First Page

2660

Last Page

2670

DOI

10.3382/ps.2007-00503

Abstract

The influence of NaHCO3 and citric acid on the antibacterial activity of apo-ovotransferrin in model systems and ham was investigated. The antibacterial activity of 20 mg/mL of ovotransferrin solution with added NaHCO3 (0, 25, 50, or 100 mM) or citric acid (0.25 or 0.5%) was evaluated against Escherichia coli O157:H7 and Listeria monocytogenes in brain heart infusion broth. The antimicrobial activity of ovotransferrin saturated with Fe2+ or Zn2+ against both pathogens was also measured. In addition, ovotransferrin solutions containing either 100 mM NaHCO3 or 0.5% citric acid were applied to commercial hams inoculated with E. coli O157:H7 or L. monocytogenes and stored at 4°C for 4 wk. The antimicrobial activity of ovotransferrin increased as the concentration of added NaHCO3 increased. Sodium bicarbonate (100 mM) significantly improved the antibacterial activity of ovotransferrin against E. coli O157:H7 and L. monocytogenes. Citric acid (0.5%) combined with ovotransferrin resulted in a synergistic antibacterial effect against E. coli O157:H7, and L. monocytogenes was susceptible to 0.5% citric acid alone. Sodium bicarbonate diminished the strong antibacterial activity of ovotransferrin + citric acid against E. coli O157:H7, and use of sodium citrate instead of citric acid did not produce any antibacterial activity against the pathogens. The antimicrobial activity of ovotransferrin increased significantly under acidic conditions. The Zn-bound ovotransferrin prevented the growth of L. monocytogenes as detected in the apo-ovotransferrin combined with 100 mM NaHCO3 treatment, but Fe-bound ovotransferrin had little or no inhibitory activity against E. coli O157:H7 and L. monocytogenes. Ovotransferrin + 100 mM NaHCO3 did not exhibit any antibacterial activity against the 2 pathogens in commercial hams, whereas ovotransferrin + 0.5% citric acid suppressed the growth of L. monocytogenes in irradiated hams. In conclusion, combinations of ovotransferrin with NaHCO3, citric acid, or Zn2+ enhanced the antibacterial activity of ovotransferrin against E. coli and L. monocytogenes, but there are some limitations as discussed for applying ovotransferrin to meat or meat products.

Comments

This article is published as Ko, K. Y., A. F. Mendonca, and D. U. Ahn. "Influence of zinc, sodium bicarbonate, and citric acid on the antibacterial activity of ovotransferrin against Escherichia coli O157: H7 and Listeria monocytogenes in model systems and ham." Poultry science 87, no. 12 (2008): 2660-2670. doi:10.3382/ps.2007-00503.

Copyright Owner

Poultry Science Association Inc.

Language

en

File Format

application/pdf

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