Campus Units

Animal Science, Food Science and Human Nutrition

Document Type

Article

Publication Version

Published Version

Publication Date

2-1-2009

Journal or Book Title

Poultry Science

Volume

88

Issue

2

First Page

406

Last Page

414

DOI

10.3382/ps.2008-00218

Abstract

The aim of this study was to evaluate the effect of EDTA, lysozyme, or the combination of EDTA and lysozyme on the antibacterial activity of ovotransferrin against Escherichia coli O157:H7. Ovotransferrin solutions (20 mg/mL) containing 100 mM NaHCO3 (OS) with added EDTA (2.0 or 2.5 mg/mL), lysozyme (1.0, 1.5, or 2.0 mg/mL), or both were prepared. The antibacterial activities of OS, OSE (OS + EDTA), or OSL (OS + lysozyme) against E. coli O157:H7 in model systems were investigated by turbidity and viability tests. In addition, OSE, OSL, or OSEL (OS + EDTA + lysozyme) was applied to irradiated pork chops and commercial hams to determine whether the solutions had antibacterial activity on meat products. The effect of the initial cell population on the antibacterial activity of OSE, OSL, and OSEL was determined. Ethylenediaminetetraacetate at 2 mg/mL plus OS induced a reduction of approximately 3 to 4 log in viable E. coli O157:H7 cells in brain heart infusion broth media, and 1 mg/mL of lysozyme plus OS resulted in a reduction of approximately 0.5 to 1.0 log during a 36-h incubation at 35°C. However, neither OSE nor OSEL showed a significant antibacterial effect on pork chops and hams during storage at 10°C. The initial cell number in media did not affect the antibacterial activity of OSE or OSEL against E. coli O157:H7. This study demonstrates that combinations of ovotransferrin, NaHCO3, and EDTA have the potential to control E. coli O157:H7.

Comments

This article is published as Ko, K. Y., A. F. Mendoncam, H. Ismail, and D. U. Ahn. "Ethylenediaminetetraacetate and lysozyme improves antimicrobial activities of ovotransferrin against Escherichia coli O157: H7." Poultry science 88, no. 2 (2009): 406-414. doi:10.3382/ps.2008-00218.

Copyright Owner

Poultry Science Association Inc.

Language

en

File Format

application/pdf

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