Campus Units

Animal Science

Document Type

Article

Publication Version

Published Version

Publication Date

8-1-2014

Journal or Book Title

Poultry Science

Volume

93

Issue

8

First Page

2103

Last Page

2107

DOI

10.3382/ps.2013-03784

Abstract

Egg yolk phosvitin is one of the most phosphorylated proteins in nature, and thus has a strong metal-binding ability. The objective of this study was to evaluate the cytotoxic and antigenotoxic activities of phosvitin in vitro. Using the 3-[4,5-dimethythiazol-2-yl]-2,5-diphenyltetrazolium bromide (MTT) assay, the cytotoxicity of phosvitin was evaluated in human cancer cell lines of various tissue origins, including the cervix (HeLa), breast (MCF-7), stomach (AGS), lung (A549 and SK-MES-1), liver (HepG2), and larynx (Hep-2). The growth of all cancer cell lines was inhibited in a dose-dependent manner by phosvitin. Among the cancer cell lines tested, MCF-7 and SK-MES-1 were the least sensitive and HeLa, AGS, and HepG2 were the most sensitive to phosvitin. The 50% inhibition of cell viability values of phosvitin were 5.38, 11.57, 4.78, 6.98, 11.82, 3.93, and 9.97 mg/mL for HeLa, MCF-7, AGS, A549, SK-MES-1, HepG2, and Hep-2, respectively. The protective effects of phosvitin against DNA damage in human leukocytes indicated that phosvitin showed protective effects against the oxidative stress-induced DNA damages in human leukocytes. These results suggested that phosvitin has a high potential to be used as an anticancer agent for humans.

Comments

This article is published as Moon, Sun Hee, Jae Hoon Lee, Minhee Lee, Eunju Park, Dong Uk Ahn, and Hyun-Dong Paik. "Cytotoxic and antigenotoxic activities of phosvitin from egg yolk." Poultry science 93, no. 8 (2014): 2103-2107. doi:10.3382/ps.2013-03784.

Copyright Owner

Poultry Science Association Inc.

Language

en

File Format

application/pdf

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