Document Type
Article
Publication Version
Published Version
Publication Date
5-2007
Journal or Book Title
Journal of the American Chemical Society
Volume
129
Issue
21
First Page
6684
Last Page
6685
DOI
10.1021/ja071158n
Abstract
Labdane-related diterpenoids are a large group of over 5,000 natural products whose biosynthesis typically proceeds through a labdadienyl/copalyl diphosphate (CPP) intermediate to a further cyclized and/or rearranged hydrocarbon diterpene en route to more elaborated compounds. Here we report a modular approach for facile biosynthesis of labdane-related diterpenes wherein base pGGxC vectors capable of introducing bacterial production of any one of the three common stereoisomers of CPP can be co-introduced with diterpene synthases that convert these CPP intermediates to specific diterpene hydrocarbon skeletal structures. The utility of this approach is demonstrated by individually engineering E. coli to produce any one of eight different diterpene skeletal structures, which collectively serve as precursors to literally thousands of distinct natural products.
Rights
One-time permission is granted only for the use specified in your request. No additional uses are granted (such as derivative works or other editions). For any other uses, please submit a new request.
Copyright Owner
American Chemical Society
Copyright Date
2007
Language
en
File Format
application/pdf
Recommended Citation
Cyr, Anthony; Wilderman, P. Ross; Determan, Mara K.; and Peters, Reuben J., "A Modular Approach for Facile Biosynthesis of Labdane-Related Diterpenes" (2007). Biochemistry, Biophysics and Molecular Biology Publications. 10.
https://lib.dr.iastate.edu/bbmb_ag_pubs/10
supplemental material
Included in
Biochemistry, Biophysics, and Structural Biology Commons, Natural Products Chemistry and Pharmacognosy Commons
Comments
Reprinted with permission from Journal of the American Chemical Society 129 (2007): 6684, doi:10.1021/ja071158n. Copyright 2007 American Chemical Society.