Campus Units
Biochemistry, Biophysics and Molecular Biology, Roy J. Carver Department of, Chemistry, Bioinformatics and Computational Biology, Physics and Astronomy
Document Type
Article
Publication Version
Accepted Manuscript
Publication Date
2011
Journal or Book Title
Nature
Volume
470
Issue
7335
First Page
558
Last Page
562
DOI
10.1038/nature09743
Abstract
Gram-negative bacteria, such as Escherichia coli, expel toxic chemicals via tripartite efflux pumps spanning both the inner and outer membranes. The three parts are: 1) a membrane fusion protein connecting 2) a substrate-binding inner membrane transporter to 3) an outer membrane-anchored channel in the periplasmic space. A crystallographic model of this tripartite efflux complex has been unavailable simply because co-crystallization of different components of the system has proven to be extremely difficult. We previously described the crystal structures of both the inner membrane transporter CusA1 and membrane fusion protein CusB2 of the CusCBA efflux system3,4 from E. coli. We here report the co-crystal structure of the CusBA efflux complex, revealing the trimeric CusA efflux pump interacts with six CusB protomers at the upper half of the periplasmic domain. These six CusB molecules form a channel extending contiguously from the top of the pump. The affinity of the CusA and CusB interaction was found to be in the micromolar range. Finally, we predicted a three-dimensional structure of the trimeric CusC outer membrane channel, and develop a model of the tripartite efflux assemblage. This CusC3-CusB6-CusA3 model presents a 750 kDa efflux complex spanning the entire bacterial cell envelope to export Cu(I)/Ag(I) ions.
Copyright Owner
Macmillan Publishers Limited
Copyright Date
2011
Language
en
File Format
application/pdf
Recommended Citation
Su, Chih-Chia; Long, Feng; Zimmermann, Michael T.; Rajashankar, Kanagalaghatta R.; Jernigan, Robert L.; and Yu, Edward W., "Crystal structure of the CusBA heavy-metal efflux complex of Escherichia coli" (2011). Biochemistry, Biophysics and Molecular Biology Publications. 153.
https://lib.dr.iastate.edu/bbmb_ag_pubs/153
Included in
Biochemistry, Biophysics, and Structural Biology Commons, Bioinformatics Commons, Biological and Chemical Physics Commons
Comments
This is a manuscript of an article published as Su, Chih-Chia, Feng Long, Michael T. Zimmermann, Kanagalaghatta R. Rajashankar, Robert L. Jernigan, and Edward W. Yu. "Crystal structure of the CusBA heavy-metal efflux complex of Escherichia coli." Nature 470, no. 7335 (2011): 558. doi: 10.1038/nature09743. Posted with permission.