Campus Units
Biochemistry, Biophysics and Molecular Biology, Roy J. Carver Department of, Baker Center for Bioinformatics and Biological Statistics
Document Type
Article
Publication Version
Accepted Manuscript
Publication Date
4-2008
Journal or Book Title
Proteins: Structure, Function, and Bioinformatics
Volume
71
Issue
1
First Page
45
Last Page
60
DOI
10.1002/prot.21652
Abstract
We combine two methods to enable the prediction of the order in which contacts are broken under external stretching forces in single molecule experiments. These two methods are Gô-like models and elastic network models. The Gô-like models have shown remarkable success in representing many aspects of protein behavior, including the reproduction of experimental data obtained from atomic force microscopy. The simple elastic network models are often used successfully to predict the fluctuations of residues around their mean positions, comparing favorably with the experimentally measured crystallographic B-factors. The behavior of biomolecules under external forces has been demonstrated to depend principally on their elastic properties and the overall shape of their structure. We have studied in detail the muscle protein titin and green fluorescent protein and tested for ten other proteins. First, we stretch the proteins computationally by performing stochastic dynamics simulations with the Gô-like model. We obtain the force–displacement curves and unfolding scenarios of possible mechanical unfolding. We then use the elastic network model to calculate temperature factors (B-factors) and compare the slowest modes of motion for the stretched proteins and compare them with the predicted order of breaking contacts between residues in the Gô-like model. Our results show that a simple Gaussian network model is able to predict contacts that break in the next time stage of stretching. Additionally, we have found that the contact disruption is strictly correlated with the highest force exerted by the backbone on these residues. Our prediction of bond-breaking agrees well with the unfolding scenario obtained with the Gô-like model. We anticipate that this method will be a useful new tool for interpreting stretching experiments.
Copyright Owner
Wiley-Liss, Inc.
Copyright Date
2007
Language
en
File Format
application/pdf
Recommended Citation
Sulkowska, Joanna I.; Kloczkowski, Andrzej; Sen, Taner Z.; Cieplak, Marek; and Jernigan, Robert L., "Predicting the order in which contacts are broken during single molecule protein stretching experiments" (2008). Biochemistry, Biophysics and Molecular Biology Publications. 176.
https://lib.dr.iastate.edu/bbmb_ag_pubs/176
Comments
This is the peer reviewed version of the following article: Sułkowska, Joanna I., Andrzej Kloczkowski, Taner Z. Sen, Marek Cieplak, and Robert L. Jernigan. "Predicting the order in which contacts are broken during single molecule protein stretching experiments." Proteins: Structure, Function, and Bioinformatics 71, no. 1 (2008): 45-60, which has been published in final form at doi: 10.1002/prot.21652. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Self-Archiving.