Biochemistry, Biophysics and Molecular Biology, Roy J. Carver Department of
Journal or Book Title
Protein Expression and Purification
Biochemical and biophysical characterization of kinases requires large quantities of purified protein. Here we report the bacterial expression and purification of active Itk kinase domain (a Tec family kinase) using ArcticExpress cells that co-express the chaperonin system Cpn60/10 from Oleispira antarctica. We describe a simple one step MgCl2/ATP/KCl incubation procedure to remove the copurifying chaperonin impurity. Chaperonin co-purification is a common problem encountered during protein purification and the simple incubation step described here completely overcomes this problem. The approach targets the chaperonin system rather than the protein of interest and is therefore widely applicable to other protein targets.
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Joseph, Raji E. and Andreotti, Amy H., "Bacterial expression and purification of Interleukin-2 Tyrosine kinase: Single step separation of the chaperonin impurity" (2008). Biochemistry, Biophysics and Molecular Biology Publications. 190.