Campus Units

Biochemistry, Biophysics and Molecular Biology, Roy J. Carver Department of, Ames Laboratory

Document Type

Article

Publication Version

Accepted Manuscript

Publication Date

3-7-2016

Journal or Book Title

Analyst

Volume

141

Issue

5

First Page

1551

Last Page

1568

DOI

10.1039/c5an01824b

Abstract

Nucleic acid aptamers are promising alternatives to antibodies in analytics. They are generally obtained through an iterative SELEX protocol that enriches a population of synthetic oligonucleotides to a subset that can recognize the chosen target molecule specifically and avidly. A wide range of targets is recognized by aptamers. Once identified and optimized for performance, aptamers can be reproducibly synthesized and offer other key features, like small size, low cost, sensitivity, specificity, rapid response, stability, and reusability. This makes them excellent options for sensory units in a variety of analytical platforms including those with electrochemical, optical, and mass sensitive transduction detection. Many novel sensing strategies have been developed by rational design to take advantage of the tendency of aptamers to undergo conformational changes upon target/analyte binding and employing the principles of base complementarity that can drive the nucleic acid structure. Despite their many advantages over antibodies, surprisingly few aptamers have yet been integrated into commercially available analytical devices. In this review, we discuss how to select and engineer aptamers for their identified application(s), some of the challenges faced in developing aptamers for analytics and many examples of their reported successful performance as sensors in a variety of analytical platforms.

Comments

This is a manuscript of an article published as Ilgu, Muslum, and Marit Nilsen-Hamilton. "Aptamers in analytics." Analyst 141, no. 5 (2016): 1551-1568. doi: 10.1039/c5an01824b. Posted with permission.

Copyright Owner

The Royal Society of Chemistry

Language

en

File Format

application/pdf

Published Version

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