Title
Campus Units
Biochemistry, Biophysics and Molecular Biology, Roy J. Carver Department of, Ames Laboratory
Document Type
Article
Publication Version
Accepted Manuscript
Publication Date
3-7-2016
Journal or Book Title
Analyst
Volume
141
Issue
5
First Page
1551
Last Page
1568
DOI
10.1039/c5an01824b
Abstract
Nucleic acid aptamers are promising alternatives to antibodies in analytics. They are generally obtained through an iterative SELEX protocol that enriches a population of synthetic oligonucleotides to a subset that can recognize the chosen target molecule specifically and avidly. A wide range of targets is recognized by aptamers. Once identified and optimized for performance, aptamers can be reproducibly synthesized and offer other key features, like small size, low cost, sensitivity, specificity, rapid response, stability, and reusability. This makes them excellent options for sensory units in a variety of analytical platforms including those with electrochemical, optical, and mass sensitive transduction detection. Many novel sensing strategies have been developed by rational design to take advantage of the tendency of aptamers to undergo conformational changes upon target/analyte binding and employing the principles of base complementarity that can drive the nucleic acid structure. Despite their many advantages over antibodies, surprisingly few aptamers have yet been integrated into commercially available analytical devices. In this review, we discuss how to select and engineer aptamers for their identified application(s), some of the challenges faced in developing aptamers for analytics and many examples of their reported successful performance as sensors in a variety of analytical platforms.
Copyright Owner
The Royal Society of Chemistry
Copyright Date
2016
Language
en
File Format
application/pdf
Recommended Citation
Ilgu, Muslum and Nilsen-Hamilton, Marit, "Aptamers in analytics" (2016). Biochemistry, Biophysics and Molecular Biology Publications. 218.
https://lib.dr.iastate.edu/bbmb_ag_pubs/218
Included in
Biochemistry, Biophysics, and Structural Biology Commons, Genetics Commons, Molecular Genetics Commons
Comments
This is a manuscript of an article published as Ilgu, Muslum, and Marit Nilsen-Hamilton. "Aptamers in analytics." Analyst 141, no. 5 (2016): 1551-1568. doi: 10.1039/c5an01824b. Posted with permission.