Campus Units
Biochemistry, Biophysics and Molecular Biology, Roy J. Carver Department of
Document Type
Article
Publication Version
Published Version
Publication Date
8-17-2018
Journal or Book Title
Scientific Reports
Volume
8
First Page
12373
DOI
10.1038/s41598-018-30590-8
Abstract
With properties such as stability to long-term storage and amenability to repetitive use, nucleic acid aptamers are compatible with many sensing/transducing platforms intended for use in remote locations. Sensors with these properties are important for quickly identifying ebolavirus outbreaks, which frequently start in locations that lack sophisticated equipment. Soluble glycoprotein (sGP), an excellent biomarker for ebolaviruses, is produced from the same gene as the ebolavirus glycoprotein GP1,2 that decorates the surface of the viral particle and is secreted in abundance into the blood stream even during the early stages of infection. Here, we report the selection and properties of a 2′fluoro pyrimidine (2′FY)-modified RNA aptamer, 39SGP1A, that specifically binds sGP. We demonstrate by computational and biochemical analysis that the recognition motif of 39SGP1A is a novel polypyrimidine-rich sequence. Replacement of -F by -OH in the 2′ position of the ribose resulted in complete loss of affinity for sGP. The protein motif to which the aptamer binds requires an intact sGP dimer and binds to an epitope conserved between Ebola virus (EBOV) and Sudan virus (SUDV) sGP, the most divergent Ebolavirus species. This identifies 39SGP1A as an excellent option for integration on a sensor platform to detect ebolavirus infections.
Rights
Works produced by employees of the U.S. Government as part of their official duties are not copyrighted within the U.S. The content of this document is not copyrighted.
Language
en
File Format
application/pdf
Recommended Citation
Shubham, Shambhavi; Hoinka, Jan; Banerjee, Soma; Swanson, Emma; Dillard, Jacob A.; Lennemann, Nicholas J.; Przytycka, Teresa M.; Maury, Wendy; and Nilsen-Hamilton, Marit, "A 2′FY-RNA Motif Defines an Aptamer for Ebolavirus Secreted Protein" (2018). Biochemistry, Biophysics and Molecular Biology Publications. 219.
https://lib.dr.iastate.edu/bbmb_ag_pubs/219
Included in
Biochemistry Commons, Biophysics Commons, Molecular Biology Commons, Structural Biology Commons
Comments
This article is published as Shubham, Shambhavi, Jan Hoinka, Soma Banerjee, Emma Swanson, Jacob A. Dillard, Nicholas J. Lennemann, Teresa M. Przytycka, Wendy Maury, and Marit Nilsen-Hamilton. "A 2′ FY-RNA Motif Defines an Aptamer for Ebolavirus Secreted Protein." Scientific reports 8 (2018): 12373. doi: 10.1038/s41598-018-30590-8.