Campus Units

Biochemistry, Biophysics and Molecular Biology, Roy J. Carver Department of, Center for Metabolic Biology, Center for Biorenewable Chemicals

Document Type

Article

Publication Version

Published Version

Publication Date

2019

Journal or Book Title

PLoS One

Volume

14

Issue

3

First Page

e0213620

DOI

https://doi.org/10.1371/journal.pone.0213620

Abstract

Fatty acids of more than 18-carbons, generally known as very long chain fatty acids (VLCFAs) are essential for eukaryotic cell viability, and uniquely in terrestrial plants they are the precursors of the cuticular lipids that form the organism’s outer barrier to the environment. VLCFAs are synthesized by fatty acid elongase (FAE), which is an integral membrane enzyme system with multiple components. The genetic complexity of the FAE system, and its membrane association has hampered the biochemical characterization of FAE. In this study we computationally identified Zea mays genetic sequences that encode the enzymatic components of FAE and developed a heterologous expression system to evaluate their functionality. The ability of the maize components to genetically complement Saccharomyces cerevisiae lethal mutants confirmed the functionality of ZmKCS4, ZmELO1, ZmKCR1, ZmKCR2, ZmHCD and ZmECR, and the VLCFA profiles of the resulting strains were used to infer the ability of each enzyme component to determine the product profile of FAE. These characterizations indicate that the product profile of the FAE system is an attribute shared among the KCS, ELO, and KCR components of FAE.

Comments

This article is published as Campbell, Alexis A., Kenna E. Stenback, Kayla Flyckt, Trang Hoang, M. Ann DN Perera, and Basil J. Nikolau. "A single-cell platform for reconstituting and characterizing fatty acid elongase component enzymes." PloS one 14, no. 3 (2019): e0213620.

Research Data:

https://doi.org/10.25380/iastate.7635962

https://doi.org/10.25380/iastate.7635959

Creative Commons License

Creative Commons Attribution 4.0 License
This work is licensed under a Creative Commons Attribution 4.0 License.

Copyright Owner

The Authors

Language

en

File Format

application/pdf

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