Campus Units

Biochemistry, Biophysics and Molecular Biology, Roy J. Carver Department of

Document Type

Article

Publication Version

Published Version

Publication Date

2006

Journal or Book Title

Journal of Cell Science

Volume

119

Issue

11

First Page

2332

Last Page

2341

DOI

10.1242/jcs.02960

Abstract

In this study we have generated two new hypomorphic Chro alleles and analyzed the consequences of reduced Chromator protein function on polytene chromosome structure. We show that in Chro71/Chro612 mutants the polytene chromosome arms were coiled and compacted with a disruption and misalignment of band and interband regions and with numerous ectopic contacts connecting non-homologous regions. Furthermore, we demonstrate that Chromator co-localizes with the JIL-1 kinase at polytene interband regions and that the two proteins interact within the same protein complex. That both proteins are necessary and may function together is supported by the finding that a concomitant reduction in JIL-1 and Chromator function synergistically reduces viability during development. Overlay assays and deletion construct analysis suggested that the interaction between JIL-1 and Chromator is direct and that it is mediated by sequences in the C-terminal domain of Chromator and by the acidic region within the C-terminal domain of JIL-1. Taken together these findings indicate that Chromator and JIL-1 interact in an interband-specific complex that functions to establish or maintain polytene chromosome structure in Drosophila.

Comments

This article is published as Rath, Uttama, Yun Ding, Huai Deng, Hongying Qi, Xiaomin Bao, Weiguo Zhang, Jack Girton, Jørgen Johansen, and Kristen M. Johansen. "The chromodomain protein, Chromator, interacts with JIL-1 kinase and regulates the structure of Drosophila polytene chromosomes." Journal of cell science 119, no. 11 (2006): 2332-2341. doi: 10.1242/jcs.02960. Posted with permission.

Copyright Owner

The Company of Biologists Limited

Language

en

File Format

application/pdf

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