Campus Units

Biochemistry, Biophysics and Molecular Biology, Roy J. Carver Department of

Document Type

Article

Publication Version

Published Version

Publication Date

3-9-2009

Journal or Book Title

Journal of Cell Biology

Volume

184

Issue

5

First Page

647

Last Page

657

DOI

10.1083/jcb.200811012

Abstract

A putative spindle matrix has been hypothesized to mediate chromosome motion, but its existence and functionality remain controversial. In this report, we show that Megator (Mtor), the Drosophilamelanogaster counterpart of the human nuclear pore complex protein translocated promoter region (Tpr), and the spindle assembly checkpoint (SAC) protein Mad2 form a conserved complex that localizes to a nuclear derived spindle matrix in living cells. Fluorescence recovery after photobleaching experiments supports that Mtor is retained around spindle microtubules, where it shows distinct dynamic properties. Mtor/Tpr promotes the recruitment of Mad2 and Mps1 but not Mad1 to unattached kinetochores (KTs), mediating normal mitotic duration and SAC response. At anaphase, Mtor plays a role in spindle elongation, thereby affecting normal chromosome movement. We propose that Mtor/Tpr functions as a spatial regulator of the SAC, which ensures the efficient recruitment of Mad2 to unattached KTs at the onset of mitosis and proper spindle maturation, whereas enrichment of Mad2 in a spindle matrix helps confine the action of a diffusible “wait anaphase” signal to the vicinity of the spindle.

Comments

This article is published as Lince-Faria, Mariana, Stefano Maffini, Bernard Orr, Yun Ding, Cláudia Florindo, Claudio E. Sunkel, Álvaro Tavares, Jørgen Johansen, Kristen M. Johansen, and Helder Maiato. "Spatiotemporal control of mitosis by the conserved spindle matrix protein Megator." The Journal of cell biology 184, no. 5 (2009): 647-657. doi: 10.1083/jcb.200811012.

Creative Commons License

Creative Commons Attribution-Noncommercial-Share Alike 3.0 License
This work is licensed under a Creative Commons Attribution-Noncommercial-Share Alike 3.0 License.

Copyright Owner

Lince-Faria et al.

Language

en

File Format

application/pdf

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