Campus Units

Biochemistry, Biophysics and Molecular Biology, Roy J. Carver Department of

Document Type

Article

Publication Version

Accepted Manuscript

Publication Date

2-1-2018

Journal or Book Title

Organic Letters

Volume

20

Issue

4

First Page

1200

Last Page

1202

DOI

10.1021/acs.orglett.8b00121

Abstract

Biosynthesis of the complex diterpenoid antibiotic pleuromutilin relies on a bifunctional (di)terpene synthase and here site-directed mutagenesis was used to knock-out either of the two active sites. This enabled characterization of the novel ring contracted intermediate produced by the initiating class II diterpene cyclase active site. Quantum chemical calculations further indicate the importance of reactant configuration for this intriguing ring rearrangement.

Comments

This is a manuscript of an article published as Xu, Meimei, Meirong Jia, Young J. Hong, Xihou Yin, Dean J. Tantillo, Philip J. Proteau, and Reuben J. Peters. "Premutilin synthase: ring rearrangement by a class II diterpene cyclase." Organic letters 20, no. 4 (2018): 1200-1202. doi: 10.1021/acs.orglett.8b00121. Posted with permission.

Copyright Owner

American Chemical Society

Language

en

File Format

application/pdf

Published Version

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