Campus Units

Biochemistry, Biophysics and Molecular Biology, Roy J. Carver Department of

Document Type

Article

Publication Version

Published Version

Publication Date

12-2004

Journal or Book Title

Plant Physiology

Volume

136

Issue

4

First Page

4228

Last Page

4236

DOI

10.1104/pp.104.050567

Abstract

Rice (Oryza sativa) produces ent-copalyl diphosphate for both gibberellin (GA) phytohormone and defensive phytoalexin biosynthesis, raising the question of how this initial biosynthetic step is carried out for these distinct metabolic processes. Here, a functional genomics approach has been utilized to identify two disparate ent-copalyl diphosphate synthases from rice (OsCPS1ent and OsCPS2ent). Notably, it was very recently demonstrated that only one of these (OsCPS1ent) normally operates in GA biosynthesis as mutations in this gene result in severely impaired growth. Evidence is presented here strongly indicating that the other (OsCPS2ent) is involved in related secondary metabolism producing defensive phytochemicals. In particular, under appropriate conditions, OsCPS2ent mRNA is specifically induced in leaves prior to production of the corresponding phytoalexins. Thus, transcriptional control of OsCPS2ent seems to be an important means of regulating defensive phytochemical biosynthesis. Finally, OsCPS1ent is significantly more similar to the likewise GA-specific gene An1/ZmCPS1ent in maize (Zea mays) than its class II terpene synthase paralogs involved in rice secondary metabolism. Hence, we speculate that this cross-species conservation by biosynthetic process reflects derivation of related secondary metabolism from the GA primary biosynthetic pathway prior to the early divergence between the separate lineages within the cereal/grass family (Poaceae) resulting in modern rice and maize.

Comments

This article is published as Prisic, Sladjana, Meimei Xu, P. Ross Wilderman, and Reuben J. Peters. "Rice contains two disparate ent-copalyl diphosphate synthases with distinct metabolic functions." Plant physiology 136, no. 4 (2004): 4228-4236. doi: 10.1104/pp.104.050567. Copyright American Society of Plant Biologists. Posted with permission.

Copyright Owner

American Society of Plant Biologists

Language

en

File Format

application/pdf

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