Campus Units

Biochemistry, Biophysics and Molecular Biology, Roy J. Carver Department of

Document Type

Article

Publication Version

Accepted Manuscript

Publication Date

1-2017

Journal or Book Title

Nature Chemical Biology

Volume

13

First Page

69

Last Page

74

DOI

10.1038/nchembio.2232

Abstract

Gibberellins (GAs) are crucial phytohormones involved in many aspects of plant growth and development, including plant-microbe interactions, which has led to GA production by plant-associated fungi and bacteria as well. While the GA biosynthetic pathways in plants and fungi have been elucidated and found to have independently arisen through convergent evolution, little has been uncovered about GA biosynthesis in bacteria. Some nitrogen-fixing/symbiotic, legume-associated rhizobia, including Bradyrhizobium japonicum, the symbiont of soybean, and Sinorhizobium fredii, a broad-host-nodulating species, contain a putative GA biosynthetic operon/gene cluster. Through functional characterization of five unknown genes, we demonstrate that this operon encodes the enzymes necessary to produce GA9, thereby elucidating bacterial GA biosynthesis. The distinct nature of these enzymes indicates that bacteria have independently evolved a third biosynthetic pathway for GA production. Furthermore, our results also reveal a central biochemical logic that is followed in all three convergently evolved GA biosynthetic pathways.

Comments

This is a manuscript of an article published as Nett, R., Montanares, M., Marcassa, A. et al. Elucidation of gibberellin biosynthesis in bacteria reveals convergent evolution. Nat Chem Biol 13, 69–74 (2017). doi: 10.1038/nchembio.2232. Posted with permission.

Copyright Owner

Nature America, Inc., part of Springer Nature

Language

en

File Format

application/pdf

Published Version

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