Campus Units

Biochemistry, Biophysics and Molecular Biology, Roy J. Carver Department of

Document Type

Article

Publication Version

Accepted Manuscript

Publication Date

1-2016

Journal or Book Title

Phytochemistry

Volume

121

First Page

4

Last Page

10

DOI

10.1016/j.phytochem.2015.10.003

Abstract

Maize (Zea mays) produces zealexins as phytoalexins, with the inducible production of these antibiotics providing biochemical protection against fungal infection. However, the biosynthesis of these sesquiterpenoids has remained unclear. In particular, it is unclear how the olefinic precursor, (S)-β-macrocarpene produced by the characterized maize sesquiterpene synthases TPS6/11, is further elaborated to form the bioactive zealexins. The first step is likely to be conversion of carbon-15 (C15) from a methyl group to a carboxylic acid by a cytochrome P450 mono-oxygenase (CYP). In this study, CYP71Z18, whose transcription is strongly induced by fungal infection, was found to catalyze oxidation of C15 in (S)-β-macrocarpene, forming zealexin A1. The inducible transcription of CYP71Z18 matches that observed for TPS6/11 and the accumulation of zealexins, which is consistent with a role for CYP71Z18 in sesquiterpenoid phytoalexin production. This completes identification of zealexin A1 biosynthesis, and represents the initial CYP identified for the production of maize terpenoid phytoalexins.

Comments

This is a manuscript of an article published as Mao, Hongjie, Jiang Liu, Fei Ren, Reuben J. Peters, and Qiang Wang. "Characterization of CYP71Z18 indicates a role in maize zealexin biosynthesis." Phytochemistry 121 (2016): 4-10. DOI: 10.1016/j.phytochem.2015.10.003. Posted with permission.

Creative Commons License

Creative Commons Attribution-Noncommercial-No Derivative Works 4.0 License
This work is licensed under a Creative Commons Attribution-Noncommercial-No Derivative Works 4.0 License.

Copyright Owner

Elsevier Ltd.

Language

en

File Format

application/pdf

Published Version

Share

COinS