Campus Units
Biochemistry, Biophysics and Molecular Biology, Roy J. Carver Department of
Document Type
Article
Publication Version
Published Version
Publication Date
1-29-2021
Journal or Book Title
Nature Communications
Volume
12
First Page
685
DOI
10.1038/s41467-021-20959-1
Abstract
Tanshinones are the bioactive nor-diterpenoid constituents of the Chinese medicinal herb Danshen (Salvia miltiorrhiza). These groups of chemicals have the characteristic furan D-ring, which differentiates them from the phenolic abietane-type diterpenoids frequently found in the Lamiaceae family. However, how the 14,16-epoxy is formed has not been elucidated. Here, we report an improved genome assembly of Danshen using a highly homozygous genotype. We identify a cytochrome P450 (CYP71D) tandem gene array through gene expansion analysis. We show that CYP71D373 and CYP71D375 catalyze hydroxylation at carbon-16 (C16) and 14,16-ether (hetero)cyclization to form the D-ring, whereas CYP71D411 catalyzes upstream hydroxylation at C20. In addition, we discover a large biosynthetic gene cluster associated with tanshinone production. Collinearity analysis indicates a more specific origin of tanshinones in Salvia genus. It illustrates the evolutionary origin of abietane-type diterpenoids and those with a furan D-ring in Lamiaceae.
Creative Commons License
This work is licensed under a Creative Commons Attribution 4.0 License.
Copyright Owner
The Author(s)
Copyright Date
2021
Language
en
File Format
application/pdf
Recommended Citation
Ma, Ying; Cui, Guanghong; Chen, Tong; Ma, Xiaohui; Wang, Ruishan; Jin, Baolong; Yang, Jian; Kang, Liping; Tang, Jinfu; Lai, Changjiangsheng; Wang, Yanan; Zhao, Yujun; Shen, Ye; Zeng, Wen; Peters, Reuben J.; Qi, Xiaoquan; Guo, Juan; and Huang, Luqi, "Expansion within the CYP71D subfamily drives the heterocyclization of tanshinones synthesis in Salvia miltiorrhiza" (2021). Biochemistry, Biophysics and Molecular Biology Publications. 292.
https://lib.dr.iastate.edu/bbmb_ag_pubs/292
Included in
Biochemistry, Biophysics, and Structural Biology Commons, Genetics and Genomics Commons, Plant Sciences Commons
Comments
This article is published as Ma, Y., Cui, G., Chen, T. et al. Expansion within the CYP71D subfamily drives the heterocyclization of tanshinones synthesis in Salvia miltiorrhiza. Nat Commun 12, 685 (2021). doi: 10.1038/s41467-021-20959-1.