Campus Units

Biochemistry, Biophysics and Molecular Biology, Roy J. Carver Department of

Document Type

Article

Publication Version

Accepted Manuscript

Publication Date

4-2021

Journal or Book Title

Phytochemistry

Volume

184

First Page

112672

DOI

10.1016/j.phytochem.2021.112672

Abstract

All land plants (embryophytes) must contain an ent-kaurene synthase (KS), as the ability to produce this olefin from ent-copalyl diphosphate (ent-CPP) is required for phytohormone biosynthesis. These KS have frequently given rise to other class I diterpene synthases that catalyze distinct reactions for more specialized plant metabolism. Indeed, the prevalence of such gene duplication and neofunctionalization has obscured phylogenetic assignment of function. Here a pair of threonines is found to be conserved in all land plant KS involved in phytohormone biosynthesis, and their role in enzyme function investigated. Surprisingly, these threonines are not required, nor even particularly important for efficient production of ent-kaurene from ent-CPP. In addition, these threonines do not seem to affect protein structure or stability. Moreover, the absence of codon bias and positioning within an intron do not support a role in transcription or translation either. Despite their lack of apparent function, this pair of threonines are nevertheless completely conserved in all embryophyte KS from phytohormone biosynthesis. Thus, regardless of exact role, this serves as a diagnostic mark for such KS, enabling more confident distinction of these critical enzymes.

Comments

This is a manuscript of an article published as Brown, Reid, Meirong Jia, and Reuben J. Peters. "A pair of threonines mark ent-kaurene synthases for phytohormone biosynthesis." Phytochemistry 184 (2021): 112672. doi:10.1016/j.phytochem.2021.112672. Posted with permission.

Copyright Owner

Elsevier Ltd.

Language

en

File Format

application/pdf

Available for download on Saturday, April 01, 2023

Published Version

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