Campus Units

Biochemistry, Biophysics and Molecular Biology, Roy J. Carver Department of, Center for Metabolic Biology

Document Type

Article

Publication Version

Published Version

Publication Date

2-2019

Journal or Book Title

Plant Direct

Volume

3

Issue

2

First Page

e00120

DOI

10.1002/pld3.120

Abstract

Nectar is the main reward that flowers offer to pollinators to entice repeated visitation. Cucurbita pepo (squash) is an excellent model for studying nectar biology, as it has large nectaries that produce large volumes of nectar relative to most other species. Squash is also monoecious, having both female and male flowers on the same plant, which allows comparative analyses of nectary function in one individual. Here, we report the nectary transcriptomes from both female and male nectaries at four stages of floral maturation. Analysis of these transcriptomes and subsequent confirmatory experiments revealed a metabolic progression in nectaries leading from starch synthesis to starch degradation and to sucrose biosynthesis. These results are consistent with previously published models of nectar secretion and also suggest how a sucrose-rich nectar can be synthesized and secreted in the absence of active transport across the plasma membrane. Nontargeted metabolomic analyses of nectars also confidently identified 40 metabolites in both female and male nectars, with some displaying preferential accumulation in nectar of either male or female flowers. Cumulatively, this study identified gene targets for reverse genetics approaches to study nectary function, as well as previously unreported nectar metabolites that may function in plant-biotic interactions.

Comments

This article is published as Solhaug, Erik M., Rahul Roy, Elizabeth C. Chatt, Peter M. Klinkenberg, Nur‐Aziatull Mohd‐Fadzil, Marshall Hampton, Basil J. Nikolau, and Clay J. Carter. "An integrated transcriptomics and metabolomics analysis of the Cucurbita pepo nectary implicates key modules of primary metabolism involved in nectar synthesis and secretion." Plant Direct 3, no. 2 (2019): e00120. doi:10.1002/pld3.120.

Creative Commons License

Creative Commons Attribution 4.0 International License
This work is licensed under a Creative Commons Attribution 4.0 International License.

Copyright Owner

The Authors

Language

en

File Format

application/pdf

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