Campus Units

Biochemistry, Biophysics and Molecular Biology, Roy J. Carver Department of, Genetics, Development and Cell Biology, Center for Metabolic Biology

Document Type

Article

Publication Version

Published Version

Publication Date

7-2020

Journal or Book Title

Plant Physiology

Volume

183

Issue

3

First Page

840

Last Page

853

DOI

10.1104/pp.20.00241

Abstract

Plant epidermal cells express unique molecular machinery that juxtapose the assembly of intracellular lipid components and the unique extracellular cuticular lipids that are unidirectionally secreted to plant surfaces. In maize (Zea mays), mutations at the glossy2 (gl2) locus affect the deposition of extracellular cuticular lipids. Sequence-based genome scanning identified a new Gl2 homolog in the maize genome, namely Gl2-like. Both the Gl2-like and Gl2 genes are members of the BAHD superfamily of acyltransferases, with close sequence similarity to the Arabidopsis (Arabidopsis thaliana) CER2 gene. Transgenic experiments demonstrated that Gl2-like and Gl2 functionally complement the Arabidopsis cer2 mutation, with differential influences on the cuticular lipids and the lipidome of the plant, particularly affecting the longer alkyl chain acyl lipids, especially at the 32-carbon chain length. Site-directed mutagenesis of the putative BAHD catalytic HXXXDX-motif indicated that Gl2-like requires this catalytic capability to fully complement the cer2 function, but Gl2 can accomplish complementation without the need for this catalytic motif. These findings demonstrate that Gl2 and Gl2-like overlap in their cuticular lipid function, but have evolutionarily diverged to acquire nonoverlapping functions.

Comments

This article is published as Alexander, Liza Esther, Yozo Okazaki, Michael A. Schelling, Aeriel Davis, Xiaobin Zheng, Ludmila Rizhsky, Marna D. Yandeau-Nelson, Kazuki Saito, and Basil J. Nikolau. "Maize Glossy2 and Glossy2-like genes have overlapping and distinct functions in cuticular lipid deposition." Plant Physiology 183, no. 3 (2020): 840-853. doi:10.1104/pp.20.00241.

Creative Commons License

Creative Commons Attribution 4.0 International License
This work is licensed under a Creative Commons Attribution 4.0 International License.

Copyright Owner

American Society of Plant Biologists

Language

en

File Format

application/pdf

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