Campus Units

Biochemistry, Biophysics and Molecular Biology, Roy J. Carver Department of, Center for Biorenewable Chemicals, Center for Metabolic Biology

Document Type

Article

Publication Version

Published Version

Publication Date

2-2020

Journal or Book Title

Plant Physiology

Volume

182

Issue

2

First Page

756

Last Page

775

DOI

10.1104/pp.19.01246

Abstract

Arabidopsis (Arabidopsis thaliana), like most dicotyledonous plants, expresses a multicomponent, heteromeric acetyl-CoA carboxylase (htACCase), which catalyzes the generation of the malonyl-CoA precursor of de novo fatty acid biosynthesis. This enzyme consists of four catalytic subunits: biotin carboxylase (BC), carboxyltransferase (CT)-α, CT-β, and biotin carboxyl carrier protein (BCCP1 or BCCP2). By coexpressing combinations of components in a bacterial expression system, we demonstrate noncatalytic BADCs facilitate the assembly and activation of BCCP–BADC–BC subcomplexes catalyzing the bicarbonate-dependent hydrolysis of ATP, which is the first half-reaction catalyzed by the htACCase enzyme. Although BADC proteins do not directly impact formation of the CT–αβ subcomplex, the BADC-facilitated BCCP–BADC–BC subcomplex can more readily interact with the CT–αβ subcomplex to facilitate the generation of malonyl-CoA. The Arabidopsis genome encodes three BADC isoforms (BADC1, BADC2, and BADC3), and BADC2 and BADC3 (rather than BADC1), in combination with BCCP1, best support this quaternary-structural organization and catalytic activation of the htACCase enzyme. Physiological genetic studies validate these attributes as Arabidopsis double mutants singularly expressing BADC2, BADC3, or BADC1 present increasingly greater deleterious impacts on morphological and biochemical phenotypes. Specifically, plants expressing only BADC2 develop normally, plants only expressing BADC3 suffer a stunted root-growth phenotype, and plants expressing only BADC1 are embryo-lethal. The latter phenotype may also be associated with the distinct suborganelle localization of BADC1 in plastids as compared to the localization of the other two BADC homologs. These finding can inspire novel strategies to improve the biological sources of fats and oils for dietary and industrial applications.

Comments

This article is published as Shivaiah, Kiran-Kumar, Geng Ding, Bryon Upton, and Basil J. Nikolau. "Non-catalytic subunits facilitate quaternary organization of plastidic acetyl-CoA carboxylase." Plant physiology 182, no. 2 (2020): 756-775. doi: 10.1104/pp.19.01246.

Creative Commons License

Creative Commons Attribution 4.0 International License
This work is licensed under a Creative Commons Attribution 4.0 International License.

Copyright Owner

American Society of Plant Biologists

Language

en

File Format

application/pdf

Share

COinS