Campus Units
Biochemistry, Biophysics and Molecular Biology, Mechanical Engineering
Document Type
Article
Publication Version
Published Version
Publication Date
2011
Journal or Book Title
Langmuir
Volume
27
Issue
23
First Page
14696
Last Page
14702
DOI
10.1021/la202067y
Abstract
A cocaine-specific aptamer was used as a receptor molecule in a microcantilever-based surface stress sensor for detection of cocaine molecules. An interferometric technique that relies on measuring differential displacement between two microcantilevers (a sensing/reference pair) was utilized to measure the cocaine/aptamer binding induced surface stress changes. Sensing experiments were performed for different concentrations of cocaine from 25 to 500 μM in order to determine the sensor response as a function of cocaine concentration. In the lower concentration range from 25 to 100 μM, surface stress values increased proportionally to coverage of aptamer/cocaine complexes from 11 to 26 mN/m. However, as the cocaine concentration was increased beyond 100 μM, the surface stress values demonstrated a weaker dependence on the affinity complex surface coverage. On the basis of a sensitivity of 3 mN/m for the surface stress measurement, the lowest detectable threshold for the cocaine concentration is estimated to be 5 μM. Sensing cantilevers could be regenerated and reused because of reversible thermal denaturation of aptamer.
Copyright Owner
American Chemical Society
Copyright Date
2011
Language
en
File Format
application/pdf
Recommended Citation
Kang, Kyungho; Sachan, Ashish; Nilsen-Hamilton, Marit; and Shrotriya, Pranav, "Aptamer Functionalized Microcantilever Sensors for Cocaine Detection" (2011). Biochemistry, Biophysics and Molecular Biology Publications. 45.
https://lib.dr.iastate.edu/bbmb_ag_pubs/45
Included in
Biochemistry, Biophysics, and Structural Biology Commons, Biomechanical Engineering Commons
Comments
Reprinted (adapted) with permission from Langmuir, 2011, 27 (23), pp 14696–14702, doi:10.1021/la202067y. Copyright 2011 American Chemical Society.