Campus Units
Biochemistry, Biophysics and Molecular Biology
Document Type
Article
Publication Version
Accepted Manuscript
Publication Date
12-2012
Journal or Book Title
Phytochemistry
Volume
84
First Page
47
Last Page
55
DOI
10.1016/j.phytochem.2012.08.021
Abstract
Wheat (Triticum aestivum) and rice (Oryza sativa) are two of the most agriculturally important cereal crop plants. Rice is known to produce numerous diterpenoid natural products that serve as phytoalexins and/or allelochemicals. Specifically, these are labdane-related diterpenoids, derived from a characteristic labdadienyl/copalyl diphosphate (CPP), whose biosynthetic relationship to gibberellin biosynthesis is evident from the relevant expanded and functionally diverse family of ent-kaurene synthase-like (KSL) genes found in rice (OsKSL). Here we report biochemical characterization of a similarly expansive family of KSL from wheat (the TaKSLs). In particular, beyond ent-kaurene synthases (KS), wheat also contains several biochemically diversified KSLs. These react either with the ent-CPP intermediate common to gibberellin biosynthesis or with the normal stereoisomer of CPP that also is found in wheat (as demonstrated by the accompanying description of wheat CPP synthases). Comparison with a barley (Hordeum vulgare) KS indicates conservation of monocot KS, with early and continued expansion and functional diversification of KSLs in at least the small grain cereals. In addition, some of the TaKSLs that utilize normal CPP also will react with syn-CPP, echoing previous findings with the OsKSL family, with such enzymatic promiscuity/plasticity providing insight into the continuing evolution of diterpenoid metabolism in the cereal crop plant family, as well as more generally, which is discussed here.
Creative Commons License
This work is licensed under a Creative Commons Attribution-Noncommercial-No Derivative Works 4.0 License.
Copyright Owner
Elsevier Ltd
Copyright Date
2012
Language
en
File Format
application/pdf
Recommended Citation
Zhou, Ke; Xu, Meimei; Tiernan, Mollie; Xie, Qian; Toyomasu, Tomonobu; Sugawara, Chizu; Oku, Madoka; Usui, Masami; Mitsuhashi, Wataru; Chono, Makiko; and Chandler, Peter M., "Functional characterization of wheat ent-kaurene(-like) synthases indicates continuing evolution of labdane-related diterpenoid metabolism in the cereals" (2012). Biochemistry, Biophysics and Molecular Biology Publications. 84.
https://lib.dr.iastate.edu/bbmb_ag_pubs/84
Included in
Agronomy and Crop Sciences Commons, Biochemistry Commons, Molecular Biology Commons, Plant Breeding and Genetics Commons
Comments
This is the author’s version of a work that was accepted for publication in Phytochemistry. Changes resulting from the publishing process, such as peer review, editing, corrections, structural formatting, and other quality control mechanisms may not be reflected in this document. Changes may have been made to this work since it was submitted for publication. A definitive version was subsequently published in Phytochemistry, VOL 84, 2012, DOI: 10.1016/j.phytochem.2012.08.021.