Biochemistry, Biophysics and Molecular Biology
Journal or Book Title
Through site-directed mutagenesis targeted at identification of the catalytic base in the rice (Oryza sativa) syn-copalyl diphosphate synthase OsCPS4, changes to a single residue (H501) were found to induce rearrangement rather than immediate deprotonation of the initially formed bicycle, leading to production of the novel compound syn-halimadienyl diphosphate. These mutational results are combined with quantum chemical calculations to provide insight into the underlying reaction mechanism.
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American Chemical Society
Potter, Kevin C.; Jia, Meirong; Hong, Young J.; Tantillo, Dean; and Peters, Reuben J., "Product Rearrangement from Altering a Single Residue in the Rice syn-Copalyl Diphosphate Synthase" (2016). Biochemistry, Biophysics and Molecular Biology Publications. 93.