Campus Units
Biochemistry, Biophysics and Molecular Biology, Roy J. Carver Department of
Document Type
Article
Publication Version
Accepted Manuscript
Publication Date
8-15-2010
Journal or Book Title
Proteins
Volume
78
Issue
11
First Page
2417
Last Page
2432
DOI
10.1002/prot.22751
Abstract
The structures and mechanism of action of many terpene cyclases are known, but there are no structures of diterpene cyclases. Here, we propose structural models based on bioinformatics, sitedirected mutagenesis, domain swapping, enzyme inhibition and spectroscopy that help explain the nature of diterpene cyclase structure, function, and evolution. Bacterial diterpene cyclases contain ~20 α-helices and the same conserved “QW” and DxDD motifs as in triterpene cyclases, indicating the presence of a βγ barrel structure. Plant diterpene cyclases have a similar catalytic motif and βγ-domain structure together with a third, α-domain, forming an αβγ structure, and in H+-initiated cyclases, there is an EDxxD-like Mg2+/diphosphate binding motif located in the γ- domain. The results support a new view of terpene cyclase structure and function and suggest evolution from ancient (βγ) bacterial triterpene cyclases to (βγ) bacterial and thence to (αβγ) plant
Copyright Owner
Wiley-Liss, Inc.
Copyright Date
2010
Language
en
File Format
application/pdf
Recommended Citation
Cao, Rong; Zhang, Yonghui; Mann, Francis M.; Huang, Cancan; Mukkamala, Dushyant; Hudock, Michael P.; Mead, Matthew; Prisic, Sladjana; Wang, Ke; Lin, Fu-Yang; Chang, Ting-Kai; Peters, Reuben; and Oldfield, Eric, "Diterpene cyclases and the nature of the isoprene fold" (2010). Biochemistry, Biophysics and Molecular Biology Publications. 99.
https://lib.dr.iastate.edu/bbmb_ag_pubs/99
Included in
Biochemistry, Biophysics, and Structural Biology Commons, Bioinformatics Commons, Genetics and Genomics Commons
Comments
This is the peer reviewed version of the following article: Cao, R., Zhang, Y., Mann, F. M., Huang, C., Mukkamala, D., Hudock, M. P., Mead, M. E., Prisic, S., Wang, K., Lin, F.-Y., Chang, T.-K., Peters, R. J. and Oldfield, E. (2010), Diterpene cyclases and the nature of the isoprene fold. Proteins, 78: 2417–2432, which has been published in final form at doi:10.1002/prot.22751. This article may be used for non-commercial purposes in accordance With Wiley Terms and Conditions for self-archiving.