Campus Units

Biomedical Sciences, Veterinary Clinical Sciences, Zoology

Document Type

Article

Publication Version

Published Version

Publication Date

10-2003

Journal or Book Title

Investigative Ophthalmology & Visual Science

Volume

44

Issue

10

First Page

4337

Last Page

4346

DOI

10.1167/iovs.03-0015

Abstract

purpose. To develop an inducible mouse model of glaucoma.

methods. An obstruction of aqueous humor outflow in adult C57BL6/J mice was induced by combined injection of indocyanine green (ICG) dye into the anterior chamber and diode laser treatment. To evaluate intraocular pressure (IOP), tonometry was performed with a modified Goldmann tonometer. The function of the retina was evaluated with electroretinography (ERG).

results. IOP was significantly elevated in surgical eyes compared with control eyes: before surgery, 15.2 ± 0.6 mm Hg; 10 days after surgery, 33.6 ± 1.5 mm Hg (P < 0.001); and 30 days after surgery, 27.4 ± 1.2 mm Hg (P < 0.001). However, 60 days after surgery, IOP in the surgical eyes decreased to 19.5 ± 0.9 mm Hg and was not significantly different compared with control eyes (control, 17.3 ± 0.7 mm Hg; P = 0.053). ERG amplitudes, expressed as a ratio (surgical/control), were decreased in surgical eyes. The amplitudes for b-wave were: before surgery, 107.6% ± 4.6%; 28 days after surgery, 61% ± 4% (P < 0.001); and 56 days after surgery, 62% ± 5.6% (P < 0.001). Oscillatory potentials were the most dramatically affected: before surgery, 108.6% ± 6.7%; 28 days after surgery, 57.5% ± 5% (P < 0.01); and 56 days after surgery, 57% ± 8.5% (P < 0.001). Amplitudes of the a-waves had relatively smaller but still significant deficits: before surgery, 105.8% ± 6.9%; 28 days after surgery, 72.2% ± 5.4% (P < 0.01); and 56 days after surgery, 79.8% ± 11.0% (P < 0.01). Histologic analysis of the surgical eyes revealed development of anterior synechia, loss of retinal ganglion cells (RGCs), and thinning of all retinal layers. Electron microscopy of optic nerve cross sections revealed swelling and degeneration of the large diameter axons and gliosis.

conclusions. Diode laser treatment of ICG saturated episcleral veins causes a chronic elevation of IOP and sustained ERG deficits.

Comments

This article is from Investigative Ophthalmology & Visual Science 44 (2003): 4337, doi: 10.1167/iovs.03-0015. Posted with permission.

Rights

This article is made available under a Creative Commons Attribution Non-Commercial No Derivatives License. For more information, please visit: http://creativecommons.org/licenses/bync-nd/4.0/legalcode

Copyright Owner

Association for Research in Vision and Ophthalmology

Language

en

File Format

application/pdf

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