Campus Units
Biomedical Sciences
Document Type
Article
Publication Version
Published Version
Publication Date
11-2013
Journal or Book Title
Eukaryotic Cell
Volume
12
Issue
11
First Page
1433
Last Page
1438
DOI
10.1128/EC.00150-13
Abstract
Entamoeba histolytica is the causative agent of amoebic dysentery, a worldwide protozoal disease that results in approximately 100,000 deaths annually. The virulence of E. histolytica may be due to interactions with the host bacterial flora, whereby trophozoites engulf colonic bacteria as a nutrient source. The engulfment process depends on trophozoite recognition of bacterial epitopes that activate phagocytosis pathways. E. histolytica GPCR-1 (EhGPCR-1) was previously recognized as a putative G-protein-coupled receptor (GPCR) used by Entamoeba histolytica during phagocytosis. In the present study, we attempted to characterize EhGPCR-1 by using heterologous GPCR expression in Saccharomyces cerevisiae. We discovered that bacterial lipopolysaccharide (LPS) is an activator of EhGPCR-1 and that LPS stimulates EhGPCR-1 in a concentration-dependent manner. Additionally, we demonstrated that Entamoeba histolytica prefers to engulf bacteria with intact LPS and that this engulfment process is sensitive to suramin, which prevents the interactions of GPCRs and G-proteins. Thus, EhGPCR-1 is an LPS-recognizing GPCR that is a potential drug target for treatment of amoebiasis, especially considering the well-established drug targeting to GPCRs.
Copyright Owner
American Society for Microbiology
Copyright Date
2016
Language
en
File Format
application/pdf
Recommended Citation
Brewer, Matthew T.; Agbedanu, Prince N.; Zamanian, Mostafa; Day, Timothy A.; and Carlson, Steve A., "Evidence for a Bacterial Lipopolysaccharide-Recognizing G-ProteinCoupled Receptor in the Bacterial Engulfment by Entamoeba histolytica" (2013). Biomedical Sciences Publications. 30.
https://lib.dr.iastate.edu/bms_pubs/30
Comments
This article is from Eukaryotic Cell 12 (2013): 1433–1438, doi:10.1128/EC.00150-13. Posted with permision.