Campus Units
Biomedical Sciences, Veterinary Pathology
Document Type
Article
Publication Version
Published Version
Publication Date
2016
Journal or Book Title
Scientific Reports
Volume
6
First Page
20193
DOI
10.1038/srep20193
Abstract
Spinal muscular atrophy (SMA) is caused by low levels of survival motor neuron (SMN), a multifunctional protein essential for higher eukaryotes. While SMN is one of the most scrutinized proteins associated with neurodegeneration, its gender-specific role in vertebrates remains unknown. We utilized a mild SMA model (C/C model) to examine the impact of low SMN on growth and development of mammalian sex organs. We show impaired testis development, degenerated seminiferous tubules, reduced sperm count and low fertility in C/C males, but no overt sex organ phenotype in C/C females. Underscoring an increased requirement for SMN expression, wild type testis showed extremely high levels of SMN protein compared to other tissues. Our results revealed severe perturbations in pathways critical to C/C male reproductive organ development and function, including steroid biosynthesis, apoptosis, and spermatogenesis. Consistent with enhanced apoptosis in seminiferous tubules of C/C testes, we recorded a drastic increase in cells with DNA fragmentation. SMN was expressed at high levels in adult C/C testis due to an adult-specific splicing switch, but could not compensate for low levels during early testicular development. Our findings uncover novel hallmarks of SMA disease progression and link SMN to general male infertility.
Rights
This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/
Copyright Owner
Ottesen, et al.
Copyright Date
2016
Language
en
File Format
application/pdf
Recommended Citation
Ottesen, Eric W.; Howell, Matthew D.; Singh, Natalia N.; Seo, Joonbae; Whitley, Elizabeth M.; and Singh, Ravindra N., "Severe impairment of male reproductive organ development in a low SMN expressing mouse model of spinal muscular atrophy" (2016). Biomedical Sciences Publications. 9.
https://lib.dr.iastate.edu/bms_pubs/9
Included in
Other Animal Sciences Commons, Veterinary Anatomy Commons, Veterinary Pathology and Pathobiology Commons, Veterinary Physiology Commons
Comments
This article is from Scientific Reports 6 (2016): 20193, doi:10.1038/srep20193. Posted with permission.