Document Type

Article

Publication Date

6-2001

Journal or Book Title

Plant Physiology

Volume

126

Issue

2

First Page

564

Last Page

574

DOI

10.​1104/​pp.​126.​2.​564

Abstract

A functional analysis of the role of glutathione in protecting plants from environmental stress was undertaken by studying Arabidopsis that had been genetically modified to have altered glutathione levels. The steady-state glutathione concentration in Arabidopsis plants was modified by expressing the cDNA for γ-glutamyl-cysteine synthetase (GSH1) in both the sense and antisense orientation. The resulting plants had glutathione levels that ranged between 3% and 200% of the level in wild-type plants. Arabidopsis plants with low glutathione levels were hypersensitive to Cd due to the limited capacity of these plants to make phytochelatins. Plants with the lowest levels of reduced glutathione (10% of wild type) were sensitive to as little as 5 μM Cd, whereas those with 50% wild-type levels required higher Cd concentrations to inhibit growth. Elevating glutathione levels did not increase metal resistance. It is interesting that the plants with low glutathione levels were also less able to accumulate anthocyanins supporting a role for glutathione S-transferases for anthocyanin formation or for the vacuolar localization and therefore accumulation of these compounds. Plants with less than 5% of wild-type glutathione levels were smaller and more sensitive to environmental stress but otherwise grew normally.

Comments

This article is from Plant Physiology 126, no. 2 (June 2001): 564–574, doi:10.1104/pp.126.2.564.

Copyright Owner

American Society of Plant Biologists

Language

en

File Format

application/pdf

Included in

Botany Commons

Share

COinS