Campus Units

Botany

Document Type

Article

Publication Version

Published Version

Publication Date

3-1991

Journal or Book Title

Plant Physiology

Volume

95

Issue

3

First Page

861

Last Page

870

DOI

10.1104/pp.95.3.861

Abstract

The protein T-URF13 (URF13) is specific to mitochondria of maize (Zea mays L.) with Texas (T) male-sterile cytoplasm and has been implicated in causing male sterility and susceptibility to T-cytoplasm-specific fungal diseases. T-URF13 was purified from isolated mitochondria from maize (line B73) with T cytoplasm by gel filtration and a quasi two-dimensional polyacrylamide gel electrophoresis system. Antibodies to the purified and denatured protein were produced in rabbits. Anti-T-URF13 antiserum was used to show that T-URF13 is in the inner membrane of mitochondria and behaves as an integral membrane protein when mitochondria are fractionated with sodium carbonate or Triton X-114. The antiserum and protein A tagged with 20-nanometer-gold particles were used to localize T-URF13 in T mitochondria by electron microscopy of sections of isolated mitochondria from etiolated shoots and sections of roots and of tapetal cells at pre-and post-degeneration stages of microsporogenesis. The microscopic study confirms that T-URF13 is specifically localized in the mitochondrial membranes of all of the T mitochondria tested, notably those in the tapetum from the meiocyte stage to the late-microspore stage. No change in the amount of labeled T-URF13 protein in the mitochondria of aging tapetal cells was detected.

Comments

This aritcle is published as Hack, Ethan, Chentao Lin, Hongyun Yang, and Harry T. Horner. "T-URF 13 protein from mitochondria of Texas male-sterile maize (Zea mays L.): Its purification and submitochondrial localization, and immunogold labeling in anther tapetum during microsporogenesis." Plant physiology 95, no. 3 (1991): 861-870. doi: 10.1104/pp.95.3.861. Copyright American Society of Plant Biologists. Posted with pemission.

Copyright Owner

American Society of Plant Biologists

Language

en

File Format

application/pdf

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