Campus Units

Agronomy, Botany, Zoology, Genetics and Genomics, Center for Plant Genomics

Document Type

Article

Publication Version

Published Version

Publication Date

5-2001

Journal or Book Title

The Plant Cell

Volume

13

Issue

5

First Page

1063

Last Page

1078

DOI

10.1105/tpc.13.5.1063

Abstract

Some plant cytoplasms express novel mitochondrial genes that cause male sterility. Nuclear genes that disrupt the accumulation of the corresponding mitochondrial gene products can restore fertility to such plants. The Texas (T) cytoplasm mitochondrial genome of maize expresses a novel protein, URF13, which is necessary for T cytoplasm–induced male sterility. Working in concert, functional alleles of two nuclear genes, rf1 and rf2, can restore fertility to T cytoplasm plants. Rf1 alleles, but not Rf2 alleles, reduce the accumulation of URF13. Hence, Rf2 differs from typical nuclear restorers in that it does not alter the accumulation of the mitochondrial protein necessary for T cytoplasm–induced male sterility. This study established that the rf2 gene encodes a soluble protein that accumulates in the mitochondrial matrix. Three independent lines of evidence establish that the RF2 protein is an aldehyde dehydrogenase (ALDH). The finding that T cytoplasm plants that are homozygous for the rf2-R213 allele are male sterile but accumulate normal amounts of RF2 protein that lacks normal mitochondrial (mt) ALDH activity provides strong evidence that rf2-encoded mtALDH activity is required to restore male fertility to T cytoplasm maize. Detailed genetic analyses have established that the rf2 gene also is required for anther development in normal cytoplasm maize. Hence, it appears that the rf2 gene was recruited recently to function as a nuclear restorer. ALDHs typically have very broad substrate specificities. Indeed, the RF2 protein is capable of oxidizing at least three aldehydes. Hence, the specific metabolic pathway(s) within which the rf2-encoded mtALDH acts remains to be discovered.

Comments

This article is published as Liu, Feng, Xiangqin Cui, Harry T. Horner, Henry Weiner, and Patrick S. Schnable. "Mitochondrial aldehyde dehydrogenase activity is required for male fertility in maize." The Plant Cell13, no. 5 (2001): 1063-1078. doi: 10.1105/tpc.13.5.1063. Copyright American Society of Plant Physiologists. Posted with permission.

Copyright Owner

American Society of Plant Physiologists

Language

en

File Format

application/pdf

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