A procedure, designated the agar disc method, has been developed at the Iowa Agricultural Experiment Station2 for the study of the contamination from churns. It consists of allowing a small amount of a special agar medium to solidify in contact with the surface to be studied, the transferring of the disc thus formed to a sterile petri dish and, finally, the counting of the colonies that develop on incubation. The usefulness of the method for the examination of churns, especially when the churns are at some distance from the laboratory3, suggests its application to the study of the contamination from metal utensils and equipment. Trials on milk cans, vats, coolers, bottlers, freezers, sanitary piping, etc., indicate that the agar disc method is readily applicable to metal surfaces.
Olsen, H. C. and Hammer, B. W.
"The agar disc method for studying the contamination from metal surfaces,"
Bulletin: Vol. 25
, Article 1.
Available at: https://lib.dr.iastate.edu/bulletin/vol25/iss300/1