A nanostructure-enabled fluorescence chip for monitoring cell secretion

Thumbnail Image
Date
2017-01-01
Authors
Che, Xiangchen
Nuhn, Jacob
Schneider, Ian
Que, Long
Major Professor
Advisor
Committee Member
Journal Title
Journal ISSN
Volume Title
Publisher
Authors
Research Projects
Organizational Units
Journal Issue
Is Version Of
Versions
Series
Department
Genetics, Development and Cell BiologyChemical and Biological Engineering
Abstract

Herein a nanostructure-enabled fluorescence chip to monitor the cell secretion is reported for the first time. The fluorescence chip is based on nanostructured aluminum oxide or nanoporous anodic aluminum oxide (Nano-AAO), which can dramatically enhance the fluorescence signal compared to coverslip glass. As a proof of concept, transforming growth factor β1 (TGF-β1) secreted directly by pancreaticstellate (iTAF) cells in isolation and in co-culture with pancreatic cancer (capan-1) cells has been monitored by the sensors using a sandwich assay. It has been found that 10 ng/ml of TGFβ1 can be readily detected in the complexity of conditioned cell media. The successful detection of the TGFβ1 secreted by iTAF cells in conditioned media indicates the possibility of the fluorescence sensors to monitor the cell secretion in real time.

Comments

This is a manuscript of a proceeding published as Che, Xiangchen, Jacob Nuhn, Ian Schneider, and Long Que. "A nanostructure-enabled fluorescence chip for monitoring cell secretion." 2017 IEEE 17th International Conference on Nanotechnology (IEEE-NANO) (2017): 1039-1042. DOI: 10.1109/NANO.2017.8117492. Posted with permission.

Description
Keywords
Citation
DOI
Copyright
Sun Jan 01 00:00:00 UTC 2017