Campus Units

Chemical and Biological Engineering, Genetics, Development and Cell Biology

Document Type

Conference Proceeding

Conference

2017 IEEE 17th International Conference on Nanotechnology (IEEE-NANO)

Publication Version

Accepted Manuscript

Link to Published Version

https://doi.org/10.1109/NANO.2017.8117492

Publication Date

2017

Journal or Book Title

2017 IEEE 17th International Conference on Nanotechnology (IEEE-NANO)

First Page

1039

Last Page

1042

Research Focus Area

Advanced and Nanostructured Materials, Health Care Technology and Biomedical Engineering

DOI

10.1109/NANO.2017.8117492

Conference Title

2017 IEEE 17th International Conference on Nanotechnology (IEEE-NANO)

Conference Date

July 25-28, 2017

City

Pittsburgh, PA

Abstract

Herein a nanostructure-enabled fluorescence chip to monitor the cell secretion is reported for the first time. The fluorescence chip is based on nanostructured aluminum oxide or nanoporous anodic aluminum oxide (Nano-AAO), which can dramatically enhance the fluorescence signal compared to coverslip glass. As a proof of concept, transforming growth factor β1 (TGF-β1) secreted directly by pancreaticstellate (iTAF) cells in isolation and in co-culture with pancreatic cancer (capan-1) cells has been monitored by the sensors using a sandwich assay. It has been found that 10 ng/ml of TGFβ1 can be readily detected in the complexity of conditioned cell media. The successful detection of the TGFβ1 secreted by iTAF cells in conditioned media indicates the possibility of the fluorescence sensors to monitor the cell secretion in real time.

Comments

This is a manuscript of a proceeding published as Che, Xiangchen, Jacob Nuhn, Ian Schneider, and Long Que. "A nanostructure-enabled fluorescence chip for monitoring cell secretion." 2017 IEEE 17th International Conference on Nanotechnology (IEEE-NANO) (2017): 1039-1042. DOI: 10.1109/NANO.2017.8117492. Posted with permission.

Rights

© 2017 IEEE. Personal use of this material is permitted. Permission from IEEE must be obtained for all other uses, in any current or future media, including reprinting/republishing this material for advertising or promotional purposes, creating new collective works, for resale or redistribution to servers or lists, or reuse of any copyrighted component of this work in other works.

Copyright Owner

IEEE

Language

en

File Format

application/pdf

Published Version

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