Document Type

Article

Publication Version

Published Version

Publication Date

2002

Journal or Book Title

Tissue Engineering

Volume

8

Issue

3

First Page

367

Last Page

378

DOI

10.1089/107632702760184646

Abstract

This article demonstrates that directional outgrowth of neurites is promoted by applying a combination of physical and chemical cues to biodegradable polymer substrates. Films of poly-D,L-lactic acid and poly(lactide-co-glycolide) were micropatterned to form grooves on substrate surfaces, using novel indirect transfer techniques developed specifically for biodegradable polymers that cannot be micropatterned directly. Laminin was selectively adsorbed in the grooves. Whole and dissociated dorsal root ganglia were seeded on the substrates and neurite outgrowth and alignment along the microgrooves were measured. The microgrooves provide physical guidance, whereas laminin provides chemical cues to the neurons. The groove depth and spacing were found to significantly influence neurite alignment. The presence of laminin was found to promote neurite adhesion and outgrowth along the grooves. Using a combination of optimized physical and chemical cues, excellent spatial control of directional neurite outgrowth, with up to 95% alignment of neurites, was obtained. The synergistic effect of physical and chemical guidance cues was found to be more effective than individual cues in promoting directional outgrowth of neurites.

Comments

This is a copy of an article published in the Tissue Engineering 2002 copyright Mary Ann Liebert, Inc.; Tissue Engineering is available online at: http://online.liebertpub.com.

Copyright Owner

Mary Ann Liebert

Language

en

File Format

application/pdf

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