Document Type
Article
Publication Version
Accepted Manuscript
Publication Date
2014
Journal or Book Title
Physical Biology
Volume
11
Issue
5
First Page
056007
DOI
10.1088/1478-3975/11/5/056007
Abstract
Cell clustering and scattering play important roles in cancer progression and tissue engineering. While the extracellular matrix (ECM) is known to control cell clustering, much of the quantitative work has focused on the analysis of clustering between cells with strong cell-cell junctions. Much less is known about how the ECM regulates cells with weak cell-cell contact. Clustering characteristics were quantified in rat adenocarcinoma cells, which form clusters on physically adsorbed collagen substrates, but not on covalently attached collagen substrates. Covalently attaching collagen inhibited desorption of collagen from the surface. While changes in proliferation rate could not explain differences seen in the clustering, changes in cell motility could. Cells plated under conditions that resulted in more clustering had a lower persistence time and slower migration rate than those under conditions that resulted in less clustering. Understanding how the ECM regulates clustering will not only impact the fundamental understanding of cancer progression, but also will guide the design of tissue engineered constructs that allow for the clustering or dissemination of cells throughout the construct.
Copyright Owner
IOP Publishing
Copyright Date
2014
Language
en
File Format
application/pdf
Recommended Citation
Hou, Yue; Rodriguez, Laura L.; Wang, Juan; and Schneider, Ian C., "Collagen attachment to the substrate controls cell clustering through migration" (2014). Chemical and Biological Engineering Publications. 259.
https://lib.dr.iastate.edu/cbe_pubs/259
Included in
Biological Engineering Commons, Cell Biology Commons, Chemical Engineering Commons, Developmental Biology Commons, Genetics Commons
Comments
This is a manuscript of an article from Physical Biology 11 (2014): 056007, doi: 10.1088/1478-3975/11/5/056007. Posted with permission.